1o7l
From Proteopedia
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| - | [[Image:1o7l.jpg|left|200px]]<br /><applet load="1o7l" size=" | + | [[Image:1o7l.jpg|left|200px]]<br /><applet load="1o7l" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1o7l, resolution 2.75Å" /> | caption="1o7l, resolution 2.75Å" /> | ||
'''MOLYBDATE-ACTIVATED FORM OF MODE FROM ESCHERICHIA COLI'''<br /> | '''MOLYBDATE-ACTIVATED FORM OF MODE FROM ESCHERICHIA COLI'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1O7L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CL, CA and MOO as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=MO1:Ca Binding Site For Chain D'>MO1</scene>. Full crystallographic information is available from [http:// | + | 1O7L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MOO:'>MOO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=MO1:Ca+Binding+Site+For+Chain+D'>MO1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7L OCA]. |
==Reference== | ==Reference== | ||
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[[Category: winged helix-turn-helix]] | [[Category: winged helix-turn-helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:54:31 2008'' |
Revision as of 07:54, 3 February 2008
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MOLYBDATE-ACTIVATED FORM OF MODE FROM ESCHERICHIA COLI
Overview
ModE is a bacterial transcriptional regulator that orchestrates many, aspects of molybdenum metabolism by binding to specific DNA sequences in a, molybdate-dependent fashion. We present the crystal structure of, Escherichia coli ModE in complex with molybdate, which was determined at, 2.75A from a merohedrally twinned crystal (twin fraction approximately, 0.30) with space group P4(3). We now have structures of ModE in both its, "switched on" (ligand-bound) and "switched off" (apo) states. Comparison, with the apo structure shows that ligand binding leads to extensive, conformational changes not only in the molybdate-binding domain, but also, in the DNA-binding domain. The most obvious difference is the loss of the, pronounced asymmetry between the two chains of the ModE dimer, which had, been a characteristic property of the apo structure. Another major change, concerns the relative orientation of the two DNA-interacting winged, helix-turn-helix motifs. Manual docking of an idealized DNA structure, suggests that this conformational change should improve DNA binding of the, activated molybdate-bound ModE.
About this Structure
1O7L is a Single protein structure of sequence from Escherichia coli with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of activated ModE reveals conformational changes involving both oxyanion and DNA-binding domains., Schuttelkopf AW, Boxer DH, Hunter WN, J Mol Biol. 2003 Feb 21;326(3):761-7. PMID:12581638
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