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1hjf
From Proteopedia
(New page: 200px<br /> <applet load="1hjf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hjf, resolution 1.60Å" /> '''ALTERATION OF THE C...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1HJF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]] with FE2 and COI as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJF OCA]]. | + | 1HJF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]] with FE2 and COI as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Sites: COI and FE. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJF OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: co-substrate selectivity]] | [[Category: co-substrate selectivity]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:34:29 2007'' |
Revision as of 10:29, 30 October 2007
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ALTERATION OF THE CO-SUBSTRATE SELECTIVITY OF DEACETOXYCEPHALOSPORIN C SYNTHASE: THE ROLE OF ARGININE-258
Overview
Deacetoxycephalosporin C synthase is an iron(II) 2-oxoglutaratedependent, oxygenase that catalyzes the oxidative ring-expansion of penicillin N to, deacetoxycephalosporin C. The wild-type enzyme is only able to efficiently, utilize 2-oxoglutarate and 2-oxoadipate as a 2-oxoacid co-substrate., Mutation of arginine 258, the side chain of which forms an electrostatic, interaction with the 5-carboxylate of the 2-oxoglutarate co-substrate, to, a glutamine residue reduced activity to about 5% of the wild-type enzyme, with 2-oxoglutarate. However, other aliphatic 2-oxoacids, which were not, co-substrates for the wild-type enzyme, were utilized by the R258Q mutant., These 2-oxoacids "rescued" catalytic activity to the level observed for, the wild-type enzyme as judged by penicillin N and G ... [(full description)]
About this Structure
1HJF is a [Single protein] structure of sequence from [Streptomyces clavuligerus] with FE2 and COI as [ligands]. Structure known Active Sites: COI and FE. Full crystallographic information is available from [OCA].
Reference
Alteration of the co-substrate selectivity of deacetoxycephalosporin C synthase. The role of arginine 258., Lee HJ, Lloyd MD, Clifton IJ, Harlos K, Dubus A, Baldwin JE, Frere JM, Schofield CJ, J Biol Chem. 2001 May 25;276(21):18290-5. Epub 2001 Feb 21. PMID:11279000
Page seeded by OCA on Tue Oct 30 12:34:29 2007
Categories: Single protein | Streptomyces clavuligerus | Baldwin, J.E. | Clifton, I.J. | Dubus, A. | Frere, J.M. | Harlos, K. | Lee, H.J. | Lloyd, M.D. | Schofield, C.J. | COI | FE2 | 2-oxoglutarate-dependent oxygenase | Alternative 2-oxoacids | Cephem antibiotic biosynthesis | Chemical cosubstrate rescue | Co-substrate selectivity
