1hjs
From Proteopedia
(New page: 200px<br /> <applet load="1hjs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hjs, resolution 1.87Å" /> '''STRUCTURE OF TWO FU...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1HJS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ | + | 1HJS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Corynascus_heterothallicus Corynascus heterothallicus]] with NAG, SO4, EPE and PEG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Arabinogalactan_endo-1,4-beta-galactosidase Arabinogalactan endo-1,4-beta-galactosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJS OCA]]. |
==Reference== | ==Reference== | ||
Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum., Le Nours J, Ryttersgaard C, Lo Leggio L, Ostergaard PR, Borchert TV, Christensen LL, Larsen S, Protein Sci. 2003 Jun;12(6):1195-204. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12761390 12761390] | Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum., Le Nours J, Ryttersgaard C, Lo Leggio L, Ostergaard PR, Borchert TV, Christensen LL, Larsen S, Protein Sci. 2003 Jun;12(6):1195-204. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12761390 12761390] | ||
| + | [[Category: Arabinogalactan endo-1,4-beta-galactosidase]] | ||
| + | [[Category: Corynascus heterothallicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Thielavia heterothallica]] | ||
[[Category: Borchert, T.V.]] | [[Category: Borchert, T.V.]] | ||
[[Category: Christensen, L.L.H.]] | [[Category: Christensen, L.L.H.]] | ||
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[[Category: thermostability]] | [[Category: thermostability]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:34:41 2007'' |
Revision as of 10:29, 30 October 2007
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STRUCTURE OF TWO FUNGAL BETA-1,4-GALACTANASES: SEARCHING FOR THE BASIS FOR TEMPERATURE AND PH OPTIMUM.
Overview
beta-1,4-Galactanases hydrolyze the galactan side chains that are part of, the complex carbohydrate structure of the pectin. They are assigned to, family 53 of the glycoside hydrolases and display significant variations, in their pH and temperature optimum and stability. Two fungal, beta-1,4-galactanases from Myceliophthora thermophila and Humicola, insolens have been cloned and heterologously expressed, and the crystal, structures of the gene products were determined. The structures are, compared to the previously only known family 53 structure of the, galactanase from Aspergillus aculeatus (AAGAL) showing approximately 56%, identity. The M. thermophila and H. insolens galactanases are thermophilic, enzymes and are most active at neutral to basic pH, whereas AAGAL is, mesophilic and most ... [(full description)]
About this Structure
1HJS is a [Single protein] structure of sequence from [Corynascus heterothallicus] with NAG, SO4, EPE and PEG as [ligands]. Active as [Arabinogalactan endo-1,4-beta-galactosidase], with EC number [3.2.1.89]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum., Le Nours J, Ryttersgaard C, Lo Leggio L, Ostergaard PR, Borchert TV, Christensen LL, Larsen S, Protein Sci. 2003 Jun;12(6):1195-204. PMID:12761390
Page seeded by OCA on Tue Oct 30 12:34:41 2007
Categories: Arabinogalactan endo-1,4-beta-galactosidase | Corynascus heterothallicus | Single protein | Borchert, T.V. | Christensen, L.L.H. | Larsen, S. | Leggio, L.Lo. | Nours, J.Le. | Ostergaard, P.R. | Ryttersgaard, C. | EPE | NAG | PEG | SO4 | 4-galactanases | Alkalophile | Beta-1 | Clan gh-a | Family 53 glycoside hydrolase | Hydrolase | Ph optimum | Thermophile | Thermostability
