1hj9
From Proteopedia
(New page: 200px<br /> <applet load="1hj9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hj9, resolution 0.95Å" /> '''ATOMIC RESOLUTION S...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1HJ9 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with SO4, CA, ANL and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJ9 OCA]]. | + | 1HJ9 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with SO4, CA, ANL and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Trypsin Trypsin]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]]. Structure known Active Site: ANL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJ9 OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Trypsin]] | ||
[[Category: Leiros, H.K.S.]] | [[Category: Leiros, H.K.S.]] | ||
[[Category: Mcsweeney, S.M.]] | [[Category: Mcsweeney, S.M.]] | ||
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[[Category: trypsin]] | [[Category: trypsin]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:35:03 2007'' |
Revision as of 10:30, 30 October 2007
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ATOMIC RESOLUTION STRUCTURES OF TRYPSIN PROVIDE INSIGHT INTO STRUCTURAL RADIATION DAMAGE
Overview
Radiation damage is an inherent problem in protein X-ray crystallography, and the process has recently been shown to be highly specific, exhibiting, features such as cleavage of disulfide bonds, decarboxylation of acidic, residues, increase in atomic B factors and increase in unit-cell volume., Reported here are two trypsin structures at atomic resolution (1.00 and, 0.95 A), the data for which were collected at a third-generation, synchrotron (ESRF) at two different beamlines. Both trypsin structures, exhibit broken disulfide bonds; in particular, the bond from Cys191 to, Cys220 is very sensitive to synchrotron radiation. The data set collected, at the most intense beamline (ID14-EH4) shows increased structural, radiation damage in terms of lower occupancies for cysteine residues, more, ... [(full description)]
About this Structure
1HJ9 is a [Single protein] structure of sequence from [Bos taurus] with SO4, CA, ANL and GOL as [ligands]. Active as [Trypsin], with EC number [3.4.21.4]. Structure known Active Site: ANL. Full crystallographic information is available from [OCA].
Reference
Atomic resolution structures of trypsin provide insight into structural radiation damage., Leiros HK, McSweeney SM, Smalas AO, Acta Crystallogr D Biol Crystallogr. 2001 Apr;57(Pt 4):488-97. PMID:11264577
Page seeded by OCA on Tue Oct 30 12:35:03 2007
