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1e2v
From Proteopedia
(New page: 200px<br /> <applet load="1e2v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e2v, resolution 1.85Å" /> '''N153Q MUTANT OF CYT...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1E2V is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]] with ACT and HEC as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E2V OCA]]. | + | 1E2V is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]] with ACT and HEC as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Sites: AC1, AC2, AC3, AC4, AC5 and AC6. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E2V OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: photosynthetic function impaired]] | [[Category: photosynthetic function impaired]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:35:55 2007'' |
Revision as of 10:31, 30 October 2007
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N153Q MUTANT OF CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII
Overview
The structure of cytochrome f includes an internal chain of five water, molecules and six hydrogen-bonding side chains, which are conserved, throughout the phylogenetic range of photosynthetic organisms from higher, plants, algae, and cyanobacteria. The in vivo electron transfer capability, of Chlamydomonas reinhardtii cytochrome f was impaired in site-directed, mutants of the conserved Asn and Gln residues that form hydrogen bonds, with water molecules of the internal chain [Ponamarev, M. V., and Cramer, W. A. (1998) Biochemistry 37, 17199-17208]. The 251-residue extrinsic, functional domain of C. reinhardtii cytochrome f was expressed in, Escherichia coli without the 35 C-terminal residues of the intact, cytochrome that contain the membrane anchor. Crystal structures were, determined ... [(full description)]
About this Structure
1E2V is a [Single protein] structure of sequence from [Chlamydomonas reinhardtii] with ACT and HEC as [ligands]. Structure known Active Sites: AC1, AC2, AC3, AC4, AC5 and AC6. Full crystallographic information is available from [OCA].
Reference
Interruption of the internal water chain of cytochrome f impairs photosynthetic function., Sainz G, Carrell CJ, Ponamarev MV, Soriano GM, Cramer WA, Smith JL, Biochemistry. 2000 Aug 8;39(31):9164-73. PMID:10924110
Page seeded by OCA on Tue Oct 30 12:35:55 2007
