1pfo
From Proteopedia
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| - | [[Image:1pfo.gif|left|200px]]<br /><applet load="1pfo" size=" | + | [[Image:1pfo.gif|left|200px]]<br /><applet load="1pfo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pfo, resolution 2.2Å" /> | caption="1pfo, resolution 2.2Å" /> | ||
'''PERFRINGOLYSIN O'''<br /> | '''PERFRINGOLYSIN O'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1PFO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Known structural/functional Site: <scene name='pdbsite=ACT:Active Site CYS Of TRP-Rich Motif'>ACT</scene>. Full crystallographic information is available from [http:// | + | 1PFO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Known structural/functional Site: <scene name='pdbsite=ACT:Active+Site+CYS+Of+TRP-Rich+Motif'>ACT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: toxin]] | [[Category: toxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:59:52 2008'' |
Revision as of 07:59, 3 February 2008
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PERFRINGOLYSIN O
Overview
The mechanisms by which proteins gain entry into membranes is a, fundamental problem in biology. Here, we present the first crystal, structure of a thiol-activated cytolysin, perfringolysin O, a member of a, large family of toxins that kill eukaryotic cells by punching holes in, their membranes. The molecule adopts an unusually elongated shape rich in, beta sheet. We have used electron microscopy data to construct a detailed, model of the membrane channel form of the toxin. The structures reveal a, novel mechanism for membrane insertion. Surprisingly, the toxin receptor, cholesterol, appears to play multiple roles: targeting, promotion of, oligomerization, triggering a membrane insertion competent form, and, stabilizing the membrane pore.
About this Structure
1PFO is a Single protein structure of sequence from Clostridium perfringens. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of a cholesterol-binding, thiol-activated cytolysin and a model of its membrane form., Rossjohn J, Feil SC, McKinstry WJ, Tweten RK, Parker MW, Cell. 1997 May 30;89(5):685-92. PMID:9182756
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