1qrz
From Proteopedia
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| - | [[Image:1qrz.gif|left|200px]]<br /><applet load="1qrz" size=" | + | [[Image:1qrz.gif|left|200px]]<br /><applet load="1qrz" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qrz, resolution 2.00Å" /> | caption="1qrz, resolution 2.00Å" /> | ||
'''CATALYTIC DOMAIN OF PLASMINOGEN'''<br /> | '''CATALYTIC DOMAIN OF PLASMINOGEN'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1QRZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Known structural/functional Sites: <scene name='pdbsite=ACA:Active Site For Chain A'>ACA</scene>, <scene name='pdbsite=ACB:Active Site For Chain B'>ACB</scene>, <scene name='pdbsite=ACC:Active Site For Chain C'>ACC</scene> and <scene name='pdbsite=ACD:Active Site For Chain D'>ACD</scene>. Full crystallographic information is available from [http:// | + | 1QRZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Known structural/functional Sites: <scene name='pdbsite=ACA:Active+Site+For+Chain+A'>ACA</scene>, <scene name='pdbsite=ACB:Active+Site+For+Chain+B'>ACB</scene>, <scene name='pdbsite=ACC:Active+Site+For+Chain+C'>ACC</scene> and <scene name='pdbsite=ACD:Active+Site+For+Chain+D'>ACD</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QRZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zymogen]] | [[Category: zymogen]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:01:33 2008'' |
Revision as of 08:01, 3 February 2008
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CATALYTIC DOMAIN OF PLASMINOGEN
Contents |
Overview
We have solved the X-ray crystal structure of the proenzyme form of the, catalytic domain of plasminogen, with the nonessential mutations M585Q, V673M, and M788L, to 2.0 A resolution. The structure presents an inactive, protease characterized by Asp740 (chymotrypsinogen 194) hydrogen bonded to, His586 (chymotrypsinogen 40), preventing proper formation of the oxyanion, hole and S1 specificity pocket. In addition, the catalytic triad residues, are misplaced relative to the active conformation adopted by serine, proteases in the chymotrypsin family. Finally, a unique form of zymogen, inactivation is observed, characterized by a "foot-in-mouth" mechanism in, which Trp761 (chymotrypsinogen 215) is folded into the S1 specificity, pocket preventing substrate binding.
Disease
Known diseases associated with this structure: Conjunctivitis, ligneous OMIM:[173350], Plasminogen Tochigi disease OMIM:[173350], Plasminogen deficiency, types I and II OMIM:[173350], Thrombophilia, dysplasminogenemic OMIM:[173350]
About this Structure
1QRZ is a Single protein structure of sequence from Homo sapiens. Known structural/functional Sites: , , and . Full crystallographic information is available from OCA.
Reference
Crystal structure of the proenzyme domain of plasminogen., Peisach E, Wang J, de los Santos T, Reich E, Ringe D, Biochemistry. 1999 Aug 24;38(34):11180-8. PMID:10460175
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