1umu
From Proteopedia
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| - | [[Image:1umu.gif|left|200px]]<br /><applet load="1umu" size=" | + | [[Image:1umu.gif|left|200px]]<br /><applet load="1umu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1umu, resolution 2.5Å" /> | caption="1umu, resolution 2.5Å" /> | ||
'''STRUCTURE DETERMINATION OF UMUD' BY MAD PHASING OF THE SELENOMETHIONYL PROTEIN'''<br /> | '''STRUCTURE DETERMINATION OF UMUD' BY MAD PHASING OF THE SELENOMETHIONYL PROTEIN'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UMU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Known structural/functional Sites: <scene name='pdbsite=CAA:The Catalytic Site Involves Residues SER 60 And LYS 97. ...'>CAA</scene> and <scene name='pdbsite=CAB:The Catalytic Site Involves Residues SER 60 And LYS 97. ...'>CAB</scene>. Full crystallographic information is available from [http:// | + | 1UMU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Known structural/functional Sites: <scene name='pdbsite=CAA:The+Catalytic+Site+Involves+Residues+SER+60+And+LYS+97.+...'>CAA</scene> and <scene name='pdbsite=CAB:The+Catalytic+Site+Involves+Residues+SER+60+And+LYS+97.+...'>CAB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UMU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: sos mutagenesis]] | [[Category: sos mutagenesis]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:02:51 2008'' |
Revision as of 08:02, 3 February 2008
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STRUCTURE DETERMINATION OF UMUD' BY MAD PHASING OF THE SELENOMETHIONYL PROTEIN
Overview
For life to be sustained, mistakes in DNA repair must be tolerated when, damage obscures the genetic information. In bacteria such as Escherichia, coli, DNA damage elicits the well regulated 'SOS response'. For the, extreme case of damage that cannot be repaired by conventional enzymes, there are proteins that allow the replication of DNA through such lesions, but with a reduction in the fidelity of replication. Essential proteins in, this mutagenic process are RecA, DNA polymerase III, UmuD, UmuD' and UmuC, (umu: UV mutagenesis). Regulation of this response involves a, RecA-mediated self-cleavage of UmuD to produce UmuD'. To understand this, system in more detail, we have determined the crystal structure of the E., coli UmuD' mutagenesis protein at 2.5 A resolution. Globular heads folded, in an unusual Beta-structure associate to form molecular dimers, and, extended amino-terminal tails associate to produce crystallized filaments., The structure provides insight into the mechanism of the self-cleavage, reaction that UmuD-like proteins undergo as part of the global SOS, response.
About this Structure
1UMU is a Single protein structure of sequence from Escherichia coli. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Structure of the UmuD' protein and its regulation in response to DNA damage., Peat TS, Frank EG, McDonald JP, Levine AS, Woodgate R, Hendrickson WA, Nature. 1996 Apr 25;380(6576):727-30. PMID:8614470
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