1un6
From Proteopedia
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| - | [[Image:1un6.jpg|left|200px]]<br /><applet load="1un6" size=" | + | [[Image:1un6.jpg|left|200px]]<br /><applet load="1un6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1un6, resolution 3.10Å" /> | caption="1un6, resolution 3.10Å" /> | ||
'''THE CRYSTAL STRUCTURE OF A ZINC FINGER- RNA COMPLEX REVEALS TWO MODES OF MOLECULAR RECOGNITION'''<br /> | '''THE CRYSTAL STRUCTURE OF A ZINC FINGER- RNA COMPLEX REVEALS TWO MODES OF MOLECULAR RECOGNITION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UN6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with ZN and MG as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1UN6 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb87_1.html Zinc Fingers]]. Known structural/functional Site: <scene name='pdbsite=BN4:Zn-Binding Site'>BN4</scene>. Full crystallographic information is available from [http:// | + | 1UN6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1UN6 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb87_1.html Zinc Fingers]]. Known structural/functional Site: <scene name='pdbsite=BN4:Zn-Binding+Site'>BN4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UN6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:02:58 2008'' |
Revision as of 08:02, 3 February 2008
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THE CRYSTAL STRUCTURE OF A ZINC FINGER- RNA COMPLEX REVEALS TWO MODES OF MOLECULAR RECOGNITION
Overview
Zinc-finger proteins of the classical Cys2His2 type are the most, frequently used class of transcription factor and account for about 3% of, genes in the human genome. The zinc-finger motif was discovered during, biochemical studies on the transcription factor TFIIIA, which regulates, the 5S ribosomal RNA genes of Xenopus laevis. Zinc-fingers mostly interact, with DNA, but TFIIIA binds not only specifically to the promoter DNA, but, also to 5S RNA itself. Increasing evidence indicates that zinc-fingers are, more widely used to recognize RNA. There have been numerous structural, studies on DNA binding, but none on RNA binding by zinc-finger proteins., Here we report the crystal structure of a three-finger complex with 61, bases of RNA, derived from the central regions of the complete nine-finger, TFIIIA-5S RNA complex. The structure reveals two modes of zinc-finger, binding, both of which differ from that in common use for DNA: first, the, zinc-fingers interact with the backbone of a double helix; and second, the, zinc-fingers specifically recognize individual bases positioned for access, in otherwise intricately folded 'loop' regions of the RNA.
About this Structure
1UN6 is a Single protein structure of sequence from Xenopus laevis with and as ligands. The following page contains interesting information on the relation of 1UN6 with [Zinc Fingers]. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of a zinc-finger-RNA complex reveals two modes of molecular recognition., Lu D, Searles MA, Klug A, Nature. 2003 Nov 6;426(6962):96-100. PMID:14603324
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