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1upk
From Proteopedia
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| - | [[Image:1upk.jpg|left|200px]]<br /><applet load="1upk" size=" | + | [[Image:1upk.jpg|left|200px]]<br /><applet load="1upk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1upk, resolution 1.85Å" /> | caption="1upk, resolution 1.85Å" /> | ||
'''CRYSTAL STRUCTURE OF MO25 IN COMPLEX WITH A C-TERMINAL PEPTIDE OF STRAD'''<br /> | '''CRYSTAL STRUCTURE OF MO25 IN COMPLEX WITH A C-TERMINAL PEPTIDE OF STRAD'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UPK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MES as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Mes Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1UPK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MES:'>MES</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Mes+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UPK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: strad]] | [[Category: strad]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:03:40 2008'' |
Revision as of 08:03, 3 February 2008
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CRYSTAL STRUCTURE OF MO25 IN COMPLEX WITH A C-TERMINAL PEPTIDE OF STRAD
Overview
Mouse protein 25 alpha (MO25 alpha) is a 40-kDa protein that, together, with the STE20-related adaptor-alpha (STRAD alpha) pseudo kinase, forms a, regulatory complex capable of stimulating the activity of the LKB1 tumor, suppressor protein kinase. The latter is mutated in the inherited, Peutz-Jeghers cancer syndrome (PJS). MO25 alpha binds directly to a, conserved Trp-Glu-Phe sequence at the STRAD alpha C terminus, markedly, enhancing binding of STRAD alpha to LKB1 and increasing LKB1 catalytic, activity. The MO25 alpha crystal structure reveals a helical repeat fold, distantly related to the Armadillo proteins. A complex with the STRAD, alpha peptide reveals a hydrophobic pocket that is involved in a unique, and specific interaction with the Trp-Glu-Phe motif, further supported by, mutagenesis studies. The data represent a first step toward structural, analysis of the LKB1-STRAD-MO25 complex, and suggests that MO25 alpha is a, scaffold protein to which other regions of STRAD-LKB1, cellular LKB1, substrates or regulatory components could bind.
About this Structure
1UPK is a Protein complex structure of sequences from Homo sapiens with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of MO25 alpha in complex with the C terminus of the pseudo kinase STE20-related adaptor., Milburn CC, Boudeau J, Deak M, Alessi DR, van Aalten DM, Nat Struct Mol Biol. 2004 Feb;11(2):193-200. Epub 2004 Jan 18. PMID:14730349
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