1upt
From Proteopedia
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| - | [[Image:1upt.jpg|left|200px]]<br /><applet load="1upt" size=" | + | [[Image:1upt.jpg|left|200px]]<br /><applet load="1upt" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1upt, resolution 1.70Å" /> | caption="1upt, resolution 1.70Å" /> | ||
'''STRUCTURE OF A COMPLEX OF THE GOLGIN-245 GRIP DOMAIN WITH ARL1'''<br /> | '''STRUCTURE OF A COMPLEX OF THE GOLGIN-245 GRIP DOMAIN WITH ARL1'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UPT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and GTP as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain G'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1UPT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GTP:'>GTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+G'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UPT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: vesicle trafficking]] | [[Category: vesicle trafficking]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:03:46 2008'' |
Revision as of 08:03, 3 February 2008
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STRUCTURE OF A COMPLEX OF THE GOLGIN-245 GRIP DOMAIN WITH ARL1
Contents |
Overview
Golgins are large coiled-coil proteins that play a role in Golgi structure, and vesicle traffic. The Arf-like GTPase Arl1 regulates the translocation, of GRIP domain-containing golgins to Golgi membranes. We report here the, 1.7 A resolution structure of human Arl1-GTP in a complex with the GRIP, domain of golgin-245. The structure reveals that the GRIP domain consists, of an S-shaped arrangement of three helices. The domain forms a homodimer, that binds two Arl1-GTPs using two helices from each monomer. The, structure is consistent with golgin-245 forming parallel coiled-coils and, suggests how Arl1-GTP/GRIP complexes interact with Golgi membranes via the, N termini of Arl1-GTP and the C-terminal tails of the GRIP domains. In, cells, bivalent association with Arl1-GTP would increase residence time of, the golgins on Golgi membranes. Despite no conservation of sequence, topology, or even helical direction, several other effectors form similar, interactions with small GTPases via a pair of alpha helices, suggesting a, common structural basis for effector recognition.
Disease
Known disease associated with this structure: Leukemia, chronic lymphatic, susceptibility to OMIM:[609351]
About this Structure
1UPT is a Protein complex structure of sequences from Homo sapiens with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis for Arl1-dependent targeting of homodimeric GRIP domains to the Golgi apparatus., Panic B, Perisic O, Veprintsev DB, Williams RL, Munro S, Mol Cell. 2003 Oct;12(4):863-74. PMID:14580338
Page seeded by OCA on Sun Feb 3 10:03:46 2008
Categories: Homo sapiens | Protein complex | Munro, S. | Panic, B. | Perisic, O. | Veprintsev, D.B. | Williams, R.L. | GTP | MG | Arl1 | G-protein | Golgi | Golgin | Golgin-245 | Grip | Grip dimer | Gtpase | Protein sorting | Vesicle trafficking
