1uqr
From Proteopedia
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| - | [[Image:1uqr.gif|left|200px]]<br /><applet load="1uqr" size=" | + | [[Image:1uqr.gif|left|200px]]<br /><applet load="1uqr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1uqr, resolution 1.7Å" /> | caption="1uqr, resolution 1.7Å" /> | ||
'''TYPE II 3-DEHYDROQUINATE DEHYDRATASE (DHQASE) FROM ACTINOBACILLUS PLEUROPNEUMONIAE'''<br /> | '''TYPE II 3-DEHYDROQUINATE DEHYDRATASE (DHQASE) FROM ACTINOBACILLUS PLEUROPNEUMONIAE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UQR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Actinobacillus_pleuropneumoniae Actinobacillus pleuropneumoniae] with SO4 and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-dehydroquinate_dehydratase 3-dehydroquinate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.10 4.2.1.10] Known structural/functional Site: <scene name='pdbsite=AC1:Trs Binding Site For Chain K'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1UQR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Actinobacillus_pleuropneumoniae Actinobacillus pleuropneumoniae] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-dehydroquinate_dehydratase 3-dehydroquinate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.10 4.2.1.10] Known structural/functional Site: <scene name='pdbsite=AC1:Trs+Binding+Site+For+Chain+K'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UQR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: shikimate pathway]] | [[Category: shikimate pathway]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:03:51 2008'' |
Revision as of 08:03, 3 February 2008
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TYPE II 3-DEHYDROQUINATE DEHYDRATASE (DHQASE) FROM ACTINOBACILLUS PLEUROPNEUMONIAE
Overview
The structure of the type II dehydroquinate dehydratase (DHQase) from, Actinobacillus pleuropneumoniae, the third enzyme of the shikimate, pathway, has been determined. Crystals diffracting to 1.7 A were obtained, in space and on earth using the counter-diffusion technique. The structure, was solved using molecular replacement and refined to high resolution. The, overall structure of the dodecameric enzyme is described and compared with, structures of DHQases from other bacteria. DHQases contain a flexible loop, that presumably closes over the active site upon substrate binding. The, enzyme can exist in an open or closed conformation. The present structure, displays the open conformation, with a sulfate anion bound in the active, site. The availability of this structure opens a route to structure-based, antibiotics targetting this pathogenic bacterium.
About this Structure
1UQR is a Single protein structure of sequence from Actinobacillus pleuropneumoniae with and as ligands. Active as 3-dehydroquinate dehydratase, with EC number 4.2.1.10 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural study of the type II 3-dehydroquinate dehydratase from Actinobacillus pleuropneumoniae., Maes D, Gonzalez-Ramirez LA, Lopez-Jaramillo J, Yu B, De Bondt H, Zegers I, Afonina E, Garcia-Ruiz JM, Gulnik S, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):463-71. Epub 2004, Feb 25. PMID:14993670
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Categories: 3-dehydroquinate dehydratase | Actinobacillus pleuropneumoniae | Single protein | Afonina, E. | Bondt, H.De. | Garcia-Ruiz, J.M. | Gonzalez-Ramirez, L.A. | Gulnik, S. | Lopez-Jaramillo, J. | Maes, D. | Yu, B. | Zegers, I. | SO4 | TRS | Aromatic amino acid biosynthesis | Lyase | Shikimate pathway
