1uqx
From Proteopedia
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| - | [[Image:1uqx.jpg|left|200px]]<br /><applet load="1uqx" size=" | + | [[Image:1uqx.jpg|left|200px]]<br /><applet load="1uqx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1uqx, resolution 1.70Å" /> | caption="1uqx, resolution 1.70Å" /> | ||
'''RALSTONIA SOLANACEARUM LECTIN (RS-IIL) IN COMPLEX WITH ALPHA-METHYLMANNOSIDE'''<br /> | '''RALSTONIA SOLANACEARUM LECTIN (RS-IIL) IN COMPLEX WITH ALPHA-METHYLMANNOSIDE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UQX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum] with MMA and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mam Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1UQX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum] with <scene name='pdbligand=MMA:'>MMA</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mam+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UQX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: sugar-binding protein]] | [[Category: sugar-binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:03:55 2008'' |
Revision as of 08:03, 3 February 2008
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RALSTONIA SOLANACEARUM LECTIN (RS-IIL) IN COMPLEX WITH ALPHA-METHYLMANNOSIDE
Overview
The plant pathogen Ralstonia solanacearum produces two lectins, each with, different affinity to fucose. We described previously the properties and, sequence of the first lectin, RSL (subunit M(r) 9.9 kDa), which is related, to fungal lectins (Sudakevitz, D., Imberty, A., and Gilboa-Garber, N., 2002, J Biochem 132: 353-358). The present communication reports the, discovery of the second one, RS-IIL (subunit M(r) 11.6 kDa), a tetrameric, lectin, with high sequence similarity to the fucose-binding lectin PA-IIL, of Pseudomonas aeruginosa. RS-IIL recognizes fucose but displays much, higher affinity to mannose and fructose, which is opposite to the, preference spectrum of PA-IIL. Determination of the crystal structure of, RS-IIL complexed with a mannose derivative demonstrates a tetrameric, structure very similar to the recently solved PA-IIL structure (Mitchell, E., et al., 2002, Nature Struct Biol 9: 918-921). Each monomer contains, two close calcium cations that mediate the binding of the monosaccharide, and explain the outstandingly high affinity to the monosaccharide ligand., The binding loop of the cations is fully conserved in RS-IIL and PA-IIL, whereas the preference for mannose versus fucose can be attributed to the, change of a three-amino-acid sequence in the 'specificity loop'.
About this Structure
1UQX is a Single protein structure of sequence from Ralstonia solanacearum with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
A new Ralstonia solanacearum high-affinity mannose-binding lectin RS-IIL structurally resembling the Pseudomonas aeruginosa fucose-specific lectin PA-IIL., Sudakevitz D, Kostlanova N, Blatman-Jan G, Mitchell EP, Lerrer B, Wimmerova M, Katcoff DJ, Imberty A, Gilboa-Garber N, Mol Microbiol. 2004 May;52(3):691-700. PMID:15101976
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