1us1

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[[Image:1us1.gif|left|200px]]<br /><applet load="1us1" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1us1.gif|left|200px]]<br /><applet load="1us1" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1us1, resolution 2.90&Aring;" />
caption="1us1, resolution 2.90&Aring;" />
'''CRYSTAL STRUCTURE OF HUMAN VASCULAR ADHESION PROTEIN-1'''<br />
'''CRYSTAL STRUCTURE OF HUMAN VASCULAR ADHESION PROTEIN-1'''<br />
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==About this Structure==
==About this Structure==
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1US1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG, CU and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Amine_oxidase_(copper-containing) Amine oxidase (copper-containing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.6 1.4.3.6] Known structural/functional Site: <scene name='pdbsite=AC1:Nag Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1US1 OCA].
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1US1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=CU:'>CU</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Amine_oxidase_(copper-containing) Amine oxidase (copper-containing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.6 1.4.3.6] Known structural/functional Site: <scene name='pdbsite=AC1:Nag+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1US1 OCA].
==Reference==
==Reference==
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[[Category: vascular adhesion protein-1]]
[[Category: vascular adhesion protein-1]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:08:42 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:04:17 2008''

Revision as of 08:04, 3 February 2008


1us1, resolution 2.90Å

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CRYSTAL STRUCTURE OF HUMAN VASCULAR ADHESION PROTEIN-1

Overview

The expression of human vascular adhesion protein-1 (hVAP-1) is induced at, sites of inflammation where extravasation of lymphocytes from blood to the, peripheral tissue occurs. We have solved the X-ray structure of hVAP-1, a, human copper amine oxidase (CAO), which is distinguished from other CAOs, in being membrane-bound. The dimer structure reveals some intriguing, features that may have fundamental roles in the adhesive and enzymatic, functions of hVAP-1, especially regarding the role of hVAP-1 in, inflammation, lymphocyte attachment, and signaling. Firstly, Leu469 at the, substrate channel may play a key role in controlling the substrate entry;, depending on its conformation, it either blocks or gives access to the, active site. Secondly, sugar units are clearly observed at two of the six, predicted N-glycosylation sites. Moreover, mutagenesis analysis showed, that all of the predicted sites were glycosylated in the protein used for, crystallization. Thirdly, the existence of a solvent-exposed RGD motif at, the entrance to each active site in hVAP-1 suggests that it may have a, functional role.

About this Structure

1US1 is a Single protein structure of sequence from Homo sapiens with , and as ligands. Active as Amine oxidase (copper-containing), with EC number 1.4.3.6 Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Crystal structure of the human vascular adhesion protein-1: unique structural features with functional implications., Airenne TT, Nymalm Y, Kidron H, Smith DJ, Pihlavisto M, Salmi M, Jalkanen S, Johnson MS, Salminen TA, Protein Sci. 2005 Aug;14(8):1964-74. PMID:16046623

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