1uvj
From Proteopedia
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| - | [[Image:1uvj.jpg|left|200px]]<br /><applet load="1uvj" size=" | + | [[Image:1uvj.jpg|left|200px]]<br /><applet load="1uvj" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1uvj, resolution 1.90Å" /> | caption="1uvj, resolution 1.90Å" /> | ||
'''THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 WITH 7NT RNA'''<br /> | '''THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 WITH 7NT RNA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UVJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=1:The Three Conserved Active Site Aspartates For Chain C'>1</scene> and <scene name='pdbsite=CA1:Mn Binding Site For Chain C'>CA1</scene>. Full crystallographic information is available from [http:// | + | 1UVJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=1:The+Three+Conserved+Active+Site+Aspartates+For+Chain+C'>1</scene> and <scene name='pdbsite=CA1:Mn+Binding+Site+For+Chain+C'>CA1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UVJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rna-dependent rna polymerase]] | [[Category: rna-dependent rna polymerase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:08:23 2008'' |
Revision as of 08:08, 3 February 2008
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THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 WITH 7NT RNA
Overview
The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA, from the three segments of the dsRNA viral genome. We have cocrystallized, RNA oligonucleotides with the polymerase, revealing the mode of binding of, RNA templates. This binding is somewhat different from that previously, seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase, complex by the addition of substrate and Mg2+ initiates a single round of, reaction within the crystal to form a dead-end complex that partially, collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we, have been able to capture the inhibited complex which shows distortion, that explains the structural basis for the inhibition of such polymerases, by Ca2+.
About this Structure
1UVJ is a Protein complex structure of sequences from [1] with as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase., Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM, Structure. 2004 Feb;12(2):307-16. PMID:14962391
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