1uvk
From Proteopedia
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| - | [[Image:1uvk.gif|left|200px]]<br /><applet load="1uvk" size=" | + | [[Image:1uvk.gif|left|200px]]<br /><applet load="1uvk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1uvk, resolution 2.45Å" /> | caption="1uvk, resolution 2.45Å" /> | ||
'''THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 DEAD-END COMPLEX'''<br /> | '''THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 DEAD-END COMPLEX'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UVK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_phi-6 Bacteriophage phi-6] with MN, MG and POP as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=CA1:Mn Binding Site For Chain C'>CA1</scene>. Full crystallographic information is available from [http:// | + | 1UVK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_phi-6 Bacteriophage phi-6] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=POP:'>POP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=CA1:Mn+Binding+Site+For+Chain+C'>CA1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UVK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rna-dependent rna polymerase]] | [[Category: rna-dependent rna polymerase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:08:23 2008'' |
Revision as of 08:08, 3 February 2008
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THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 DEAD-END COMPLEX
Overview
The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA, from the three segments of the dsRNA viral genome. We have cocrystallized, RNA oligonucleotides with the polymerase, revealing the mode of binding of, RNA templates. This binding is somewhat different from that previously, seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase, complex by the addition of substrate and Mg2+ initiates a single round of, reaction within the crystal to form a dead-end complex that partially, collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we, have been able to capture the inhibited complex which shows distortion, that explains the structural basis for the inhibition of such polymerases, by Ca2+.
About this Structure
1UVK is a Protein complex structure of sequences from Bacteriophage phi-6 with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase., Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM, Structure. 2004 Feb;12(2):307-16. PMID:14962391
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