1uvo
From Proteopedia
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| - | [[Image:1uvo.gif|left|200px]]<br /><applet load="1uvo" size=" | + | [[Image:1uvo.gif|left|200px]]<br /><applet load="1uvo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1uvo, resolution 1.85Å" /> | caption="1uvo, resolution 1.85Å" /> | ||
'''STRUCTURE OF THE COMPLEX OF PORCINE PANCREATIC ELASTASE IN COMPLEX WITH CADMIUM REFINED AT 1.85 A RESOLUTION (CRYSTAL A)'''<br /> | '''STRUCTURE OF THE COMPLEX OF PORCINE PANCREATIC ELASTASE IN COMPLEX WITH CADMIUM REFINED AT 1.85 A RESOLUTION (CRYSTAL A)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UVO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with ACT, CL, CD and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Known structural/functional Site: <scene name='pdbsite=AC1:Cd Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1UVO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=CD:'>CD</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Known structural/functional Site: <scene name='pdbsite=AC1:Cd+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UVO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:08:26 2008'' |
Revision as of 08:08, 3 February 2008
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STRUCTURE OF THE COMPLEX OF PORCINE PANCREATIC ELASTASE IN COMPLEX WITH CADMIUM REFINED AT 1.85 A RESOLUTION (CRYSTAL A)
Overview
Two series of complete and highly redundant data sets were collected at, wavelengths of 1.00 and 2.00 Angstroms on a cadmium derivative of porcine, pancreatic elastase (PPE). Radiation damage to the sample was evaluated, qualitatively by inspecting consecutive difference electron density maps, during the course of the experiment. The nature of the radiation damage, was found to be identical at both wavelengths and was localized primarily, at the four disulfide bridges of PPE, the cadmium site and the two, methionine residues. For a quantitative examination of the radiation, damage, the decrease in the peak height of the cadmium ion in various, electron density maps was exploited. Again, no significant difference in, radiation damage between the two wavelengths was observed. This can be, rationalized by considering the wavelength dependencies of the number of, diffracted photons versus the number of absorbed photons and the energy, deposited in the crystal by the latter.
About this Structure
1UVO is a Single protein structure of sequence from Sus scrofa with , , and as ligands. Active as Pancreatic elastase, with EC number 3.4.21.36 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
On the influence of the incident photon energy on the radiation damage in crystalline biological samples., Weiss MS, Panjikar S, Mueller-Dieckmann C, Tucker PA, J Synchrotron Radiat. 2005 May;12(Pt 3):304-9. Epub 2005 Apr 14. PMID:15840915
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