1uwv
From Proteopedia
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| - | [[Image:1uwv.jpg|left|200px]]<br /><applet load="1uwv" size=" | + | [[Image:1uwv.jpg|left|200px]]<br /><applet load="1uwv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1uwv, resolution 1.95Å" /> | caption="1uwv, resolution 1.95Å" /> | ||
'''CRYSTAL STRUCTURE OF RUMA, THE IRON-SULFUR CLUSTER CONTAINING E. COLI 23S RIBOSOMAL RNA 5-METHYLURIDINE METHYLTRANSFERASE'''<br /> | '''CRYSTAL STRUCTURE OF RUMA, THE IRON-SULFUR CLUSTER CONTAINING E. COLI 23S RIBOSOMAL RNA 5-METHYLURIDINE METHYLTRANSFERASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UWV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with NI, PO4, CL and SF4 as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=CAT:Po4 Binding Site For Chain A'>CAT</scene>. Full crystallographic information is available from [http:// | + | 1UWV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=SF4:'>SF4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=CAT:Po4+Binding+Site+For+Chain+A'>CAT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UWV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:08:52 2008'' |
Revision as of 08:08, 3 February 2008
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CRYSTAL STRUCTURE OF RUMA, THE IRON-SULFUR CLUSTER CONTAINING E. COLI 23S RIBOSOMAL RNA 5-METHYLURIDINE METHYLTRANSFERASE
Overview
RumA catalyzes transfer of a methyl group from S-adenosylmethionine (SAM), specifically to uridine 1939 of 23S ribosomal RNA in Escherichia coli to, yield 5-methyluridine. We determined the crystal structure of RumA at 1.95, A resolution. The protein is organized into three structural domains: The, N-terminal domain contains sequence homology to the conserved TRAM motif, and displays a five-stranded beta barrel architecture characteristic of an, oligosaccharide/oligonucleotide binding fold. The central domain contains, a [Fe(4)S(4)] cluster coordinated by four conserved cysteine residues. The, C-terminal domain displays the typical SAM-dependent methyltransferase, fold. The catalytic nucleophile Cys389 lies in a motif different from that, in DNA 5-methylcytosine methyltransferases. The electrostatic potential, surface reveals a predominately positively charged area that covers the, concave surface of the first two domains and suggests an RNA binding mode., The iron-sulfur cluster may be involved in the correct folding of the, protein or may have a role in RNA binding.
About this Structure
1UWV is a Single protein structure of sequence from Escherichia coli with , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase., Lee TT, Agarwalla S, Stroud RM, Structure. 2004 Mar;12(3):397-407. PMID:15016356
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Categories: Escherichia coli | Single protein | Agarwalla, S. | Lee, T.T. | Stroud, R.M. | CL | NI | PO4 | SF4 | Iron-sulfur cluster | Methyltransferase | Rna modification | Rna processing | Transferase
