Sandbox 35
From Proteopedia
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==Introduction== | ==Introduction== | ||
| - | [[Image:Papain_cartoon.png|200px|left|thumb|]] | + | [[Image:Papain_cartoon.png|200px|left|thumb|Cartoon Peak at Pepsin]] |
<scene name='Sandbox_35/Papain/1'>Papain</scene> finds its origin from the latex of the papaya fruit and is classified as a sulfhydryl protease. | <scene name='Sandbox_35/Papain/1'>Papain</scene> finds its origin from the latex of the papaya fruit and is classified as a sulfhydryl protease. | ||
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===Distribution of Residues=== | ===Distribution of Residues=== | ||
| + | <scene name='Sandbox_35/Papain_acid_and_basic_residues/1'>acidic and basic residues</scene> | ||
| + | |||
<scene name='Sandbox_35/Hydrophobic_papain/1'>non-polar and polar residues</scene> | <scene name='Sandbox_35/Hydrophobic_papain/1'>non-polar and polar residues</scene> | ||
Revision as of 04:44, 13 November 2011
| Please do NOT make changes to this Sandbox. Sandboxes 30-60 are reserved for use by Biochemistry 410 & 412 at Messiah College taught by Dr. Hannah Tims during Fall 2012 and Spring 2013. |
Contents |
Papain
Introduction
finds its origin from the latex of the papaya fruit and is classified as a sulfhydryl protease.
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Catalytic Mechanism
References
- ↑ [1]9PAP PDB
- ↑ Wang J, Xiang YF, Lim C. The double catalytic triad, Cys25-His159-Asp158 and Cys25-His159-Asn175, in papain catalysis: role of Asp158 and Asn175. Protein Eng. 1994 Jan;7(1):75-82. PMID:8140097
- ↑ [2] University of Maine
http://www.pdb.org/pdb/explore/explore.do?structureId=2PAD
• Show the secondary structures.
• Compare the distribution of polar residues to that of nonpolar residues.
• Highlight the active site.
• If you can find a PDB file of the enzyme that contains a pseudo-substrate (may be inhibitor), highlight it.
• Show the contacts or attractions that are present between the pseudo-substrate and the protein, and if the enzyme has multiple subunits, show the contacts between the subunits.
• Identify any other ligands that are present in the structure and the types of contacts that are present between them and the protein
http://proteopedia.org/wiki/index.php/Sandbox_55#cite_note-18 Table of contents Pictures References (cross links)
