From Proteopedia

proteopedia linkproteopedia link

Revision as of 00:21, 14 November 2011 (edit) Student (Talk | contribs) ← Previous diff		Revision as of 00:35, 14 November 2011 (edit) (undo) Student (Talk | contribs) Next diff →
Line 5:		Line 5:
	==Introduction==		==Introduction==
	[[Image:Papaya.jpg | right| thumb| ''Carica papaya'']]		[[Image:Papaya.jpg | right| thumb| ''Carica papaya'']]
-	<scene name='Sandbox_37/Main_scene/1'>Papain</scene> is a cysteine protease that is stable and active under a wide range of conditions. The enzyme present in the leaves, latex, roots, and fruit of the papaya plant(''Carica papaya'').	+	<scene name='Sandbox_37/Main_scene/1'>Papain</scene> is a cysteine protease that is stable and active under a wide range of conditions. The enzyme present in the leaves, latex, roots, and fruit of the papaya plant (''Carica papaya'').<ref>http://www.britannica.com/EBchecked/topic/441803/papain</ref> The papain proteins are synthesized as inactive precursors that become active within two minutes of the plant being wounded and the latex is expelled.<ref>http://www.worthington-biochem.com/pap/default.html</ref>
	==Structure==		==Structure==
-	<StructureSection load='9PAP' size='400' align='left' caption='Papain (9PAP)' scene=''></StructureSection>	+	<StructureSection load='9PAP' size='400' align='left' caption='Papain (9PAP)' scene=''></StructureSection> Papain consists of one polypepetide chain that is made up of 212 amino acids residues. There are three <scene name='Sandbox_37/Papain_disulfide_bonds/1'>disulfide bonds</scene> present in the enzyme that maintains the protein's structure.
	<scene name='Sandbox_37/Papain_beta-alpha/1'>secondary structure</scene>		<scene name='Sandbox_37/Papain_beta-alpha/1'>secondary structure</scene>
Line 22:		Line 22:
	<scene name='Sandbox_37/Papain_hydrogen_bonds/1'>hydrogen bonds</scene>		<scene name='Sandbox_37/Papain_hydrogen_bonds/1'>hydrogen bonds</scene>
-	<scene name='Sandbox_37/Papain_disulfide_bonds/1'>disulfide bonds</scene>
	==Mechanism==		==Mechanism==
Line 39:		Line 38:
	<ref>http://www.pdb.org/pdb/explore/remediatedSequence.do?structureId=1POP&params.chainEntityStrategyStr=all</ref>		<ref>http://www.pdb.org/pdb/explore/remediatedSequence.do?structureId=1POP&params.chainEntityStrategyStr=all</ref>
-	<ref>http://www.worthington-biochem.com/pap/default.html</ref>
	<ref>http://en.wikipedia.org/wiki/Papain</ref>		<ref>http://en.wikipedia.org/wiki/Papain</ref>
	<ref>http://www.pdb.org/pdb/explore.do?structureId=9PAP</ref>		<ref>http://www.pdb.org/pdb/explore.do?structureId=9PAP</ref>
-	<ref>http://www.britannica.com/EBchecked/topic/441803/papain</ref>
	<ref>http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/analytical-enzymes/papain.html</ref>		<ref>http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/analytical-enzymes/papain.html</ref>
	==References==		==References==
	<references />		<references />

---

Revision as of 00:35, 14 November 2011

Please do NOT make changes to this Sandbox. Sandboxes 30-60 are reserved for use by Biochemistry 410 & 412 at Messiah College taught by Dr. Hannah Tims during Fall 2012 and Spring 2013.

Contents 1 Papain 1.1 Introduction 1.2 Structure 1.3 Mechanism 1.4 Function 1.5 Inhibition 1.6 References

Papain

Introduction

is a cysteine protease that is stable and active under a wide range of conditions. The enzyme present in the leaves, latex, roots, and fruit of the papaya plant (Carica papaya).^[1] The papain proteins are synthesized as inactive precursors that become active within two minutes of the plant being wounded and the latex is expelled.^[2]

Structure

spinqualitylabelsall models Papain (9PAP) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Papain consists of one polypepetide chain that is made up of 212 amino acids residues. There are three present in the enzyme that maintains the protein's structure.

Mechanism

Function

Inhibition

^{[3] [4] [5] [6]}

References

1. ↑ http://www.britannica.com/EBchecked/topic/441803/papain
2. ↑ http://www.worthington-biochem.com/pap/default.html
3. ↑ http://www.pdb.org/pdb/explore/remediatedSequence.do?structureId=1POP&params.chainEntityStrategyStr=all
4. ↑ http://en.wikipedia.org/wiki/Papain
5. ↑ http://www.pdb.org/pdb/explore.do?structureId=9PAP
6. ↑ http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/analytical-enzymes/papain.html