1v0d
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1v0d.gif|left|200px]]<br /><applet load="1v0d" size=" | + | [[Image:1v0d.gif|left|200px]]<br /><applet load="1v0d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1v0d, resolution 2.6Å" /> | caption="1v0d, resolution 2.6Å" /> | ||
'''CRYSTAL STRUCTURE OF CASPASE-ACTIVATED DNASE (CAD)'''<br /> | '''CRYSTAL STRUCTURE OF CASPASE-ACTIVATED DNASE (CAD)'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1V0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN, MG and PB as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1V0D with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb56_1.html Caspases]]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1V0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=PB:'>PB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1V0D with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb56_1.html Caspases]]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V0D OCA]. |
==Reference== | ==Reference== | ||
| Line 27: | Line 27: | ||
[[Category: nuclease]] | [[Category: nuclease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:13:17 2008'' |
Revision as of 08:13, 3 February 2008
|
CRYSTAL STRUCTURE OF CASPASE-ACTIVATED DNASE (CAD)
Overview
CAD/DFF40 is responsible for the degradation of chromosomal DNA into, nucleosomal fragments and subsequent chromatin condensation during, apoptosis. It exists as an inactive complex with its inhibitor ICAD/DFF45, in proliferating cells but becomes activated upon cleavage of ICAD/DFF45, into three domains by caspases in dying cells. The molecular mechanism, underlying the control and activation of CAD/DFF40 was unknown. Here, the, crystal structure of activated CAD/DFF40 reveals that it is a pair of, molecular scissors with a deep active-site crevice that appears ideal for, distinguishing internucleosomal DNA from nucleosomal DNA. Ensuing studies, show that ICAD/DFF45 sequesters the nonfunctional CAD/DFF40 monomer and is, also able to disassemble the functional CAD/DFF40 dimer. This capacity, requires the involvement of the middle domain of ICAD/DFF45, which by, itself cannot remain bound to CAD/DFF40 due to low binding affinity for, the enzyme. Thus, the consequence of the caspase-cleavage of ICAD/DFF45 is, a self-assembly of CAD/DFF40 into the active dimer.
About this Structure
1V0D is a Single protein structure of sequence from Mus musculus with , and as ligands. The following page contains interesting information on the relation of 1V0D with [Caspases]. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural mechanism for inactivation and activation of CAD/DFF40 in the apoptotic pathway., Woo EJ, Kim YG, Kim MS, Han WD, Shin S, Robinson H, Park SY, Oh BH, Mol Cell. 2004 May 21;14(4):531-9. PMID:15149602
Page seeded by OCA on Sun Feb 3 10:13:17 2008
Categories: Caspases | Mus musculus | Single protein | Han, W.D. | Kim, M.S. | Kim, Y.G. | Oh, B.H. | Shin, S. | Woo, E.J. | MG | PB | ZN | Caspase-activated dnase | Hydrolase | Nuclease
