1w1q
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1w1q.gif|left|200px]]<br /><applet load="1w1q" size=" | + | [[Image:1w1q.gif|left|200px]]<br /><applet load="1w1q" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w1q, resolution 1.80Å" /> | caption="1w1q, resolution 1.80Å" /> | ||
'''PLANT CYTOKININ DEHYDROGENASE IN COMPLEX WITH ISOPENTENYLADENINE'''<br /> | '''PLANT CYTOKININ DEHYDROGENASE IN COMPLEX WITH ISOPENTENYLADENINE'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1W1Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays] with NAG, FAD and ZIP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] Known structural/functional Site: <scene name='pdbsite=AC1:Nag Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1W1Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=ZIP:'>ZIP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] Known structural/functional Site: <scene name='pdbsite=AC1:Nag+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W1Q OCA]. |
==Reference== | ==Reference== | ||
| Line 25: | Line 25: | ||
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:18:10 2008'' |
Revision as of 08:18, 3 February 2008
|
PLANT CYTOKININ DEHYDROGENASE IN COMPLEX WITH ISOPENTENYLADENINE
Overview
Cytokinins form a diverse class of compounds that are essential for plant, growth. Cytokinin dehydrogenase has a major role in the control of the, levels of these plant hormones by catalysing their irreversible oxidation., The crystal structure of Zea mays cytokinin dehydrogenase displays the, same two-domain topology of the flavoenzymes of the vanillyl-alcohol, oxidase family but its active site cannot be related to that of any other, family member. The X-ray analysis reveals a bipartite architecture of the, catalytic centre, which consists of a funnel-shaped region on the protein, surface and an internal cavity lined by the flavin ring. A pore with, diameter of about 4A connects the two active-site regions. Snapshots of, two critical steps along the reaction cycle were obtained through the, structural analysis of the complexes with a slowly reacting substrate and, the reaction product, which correspond to the states immediately before, (Michaelis complex) and after (product complex) oxidation has taken place., The substrate displays a "plug-into-socket" binding mode that seals the, catalytic site and precisely positions the carbon atom undergoing, oxidation in close contact with the reactive locus of the flavin. A, polarising H-bond between the substrate amine group and an Asp-Glu pair, may facilitate oxidation. Substrate to product conversion results in small, atomic movements, which lead to a planar conformation of the reaction, product allowing double-bond conjugation. These features in the mechanism, of amine recognition and oxidation differ from those observed in other, flavin-dependent amine oxidases.
About this Structure
1W1Q is a Single protein structure of sequence from Zea mays with , and as ligands. Active as Cytokinin dehydrogenase, with EC number 1.5.99.12 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structures of Michaelis and product complexes of plant cytokinin dehydrogenase: implications for flavoenzyme catalysis., Malito E, Coda A, Bilyeu KD, Fraaije MW, Mattevi A, J Mol Biol. 2004 Aug 27;341(5):1237-49. PMID:15321719
Page seeded by OCA on Sun Feb 3 10:18:10 2008
Categories: Cytokinin dehydrogenase | Single protein | Zea mays | Malito, E. | Mattevi, A. | FAD | NAG | ZIP | Cytokinin | Fad | Flavin | Flavoprotein | Oxidoreductase
