1w2b
From Proteopedia
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| - | [[Image:1w2b.gif|left|200px]]<br /><applet load="1w2b" size=" | + | [[Image:1w2b.gif|left|200px]]<br /><applet load="1w2b" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w2b, resolution 3.50Å" /> | caption="1w2b, resolution 3.50Å" /> | ||
'''TRIGGER FACTOR RIBOSOME BINDING DOMAIN IN COMPLEX WITH 50S'''<br /> | '''TRIGGER FACTOR RIBOSOME BINDING DOMAIN IN COMPLEX WITH 50S'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W2B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] with MG, NA, K, CL and CD as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cl Binding Site For Chain 0'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1W2B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+0'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2B OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rna-binding]] | [[Category: rna-binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:18:24 2008'' |
Revision as of 08:18, 3 February 2008
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TRIGGER FACTOR RIBOSOME BINDING DOMAIN IN COMPLEX WITH 50S
Overview
During protein biosynthesis, nascent polypeptide chains that emerge from, the ribosomal exit tunnel encounter ribosome-associated chaperones, which, assist their folding to the native state. Here we present a 2.7 A crystal, structure of Escherichia coli trigger factor, the best-characterized, chaperone of this type, together with the structure of its, ribosome-binding domain in complex with the Haloarcula marismortui large, ribosomal subunit. Trigger factor adopts a unique conformation resembling, a crouching dragon with separated domains forming the amino-terminal, ribosome-binding 'tail', the peptidyl-prolyl isomerase 'head', the, carboxy-terminal 'arms' and connecting regions building up the 'back'., From its attachment point on the ribosome, trigger factor projects the, extended domains over the exit of the ribosomal tunnel, creating a, protected folding space where nascent polypeptides may be shielded from, proteases and aggregation. This study sheds new light on our understanding, of co-translational protein folding, and suggests an unexpected mechanism, of action for ribosome-associated chaperones.
About this Structure
1W2B is a Protein complex structure of sequences from Escherichia coli and Haloarcula marismortui with , , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Trigger factor in complex with the ribosome forms a molecular cradle for nascent proteins., Ferbitz L, Maier T, Patzelt H, Bukau B, Deuerling E, Ban N, Nature. 2004 Sep 30;431(7008):590-6. Epub 2004 Aug 29. PMID:15334087
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Categories: Escherichia coli | Haloarcula marismortui | Protein complex | Ban, N. | Bukau, B. | Deuerling, E. | Ferbitz, L. | Maier, T. | Patzelt, H. | CD | CL | K | MG | NA | Chaperone | Cotranslational folding | Nascent chain | Ribosomal protein | Ribosome | Ribosome associated factors | Rna-binding
