1w36
From Proteopedia
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| - | [[Image:1w36.gif|left|200px]]<br /><applet load="1w36" size=" | + | [[Image:1w36.gif|left|200px]]<br /><applet load="1w36" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w36, resolution 3.1Å" /> | caption="1w36, resolution 3.1Å" /> | ||
'''RECBCD:DNA COMPLEX'''<br /> | '''RECBCD:DNA COMPLEX'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W36 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Exodeoxyribonuclease_V Exodeoxyribonuclease V], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.5 3.1.11.5] Known structural/functional Site: <scene name='pdbsite=AC1:Ca Binding Site For Chain E'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1W36 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Exodeoxyribonuclease_V Exodeoxyribonuclease V], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.5 3.1.11.5] Known structural/functional Site: <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+E'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W36 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: recombination]] | [[Category: recombination]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:18:45 2008'' |
Revision as of 08:18, 3 February 2008
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RECBCD:DNA COMPLEX
Overview
RecBCD is a multi-functional enzyme complex that processes DNA ends, resulting from a double-strand break. RecBCD is a bipolar helicase that, splits the duplex into its component strands and digests them until, encountering a recombinational hotspot (Chi site). The nuclease activity, is then attenuated and RecBCD loads RecA onto the 3' tail of the DNA. Here, we present the crystal structure of RecBCD bound to a DNA substrate. In, this initiation complex, the DNA duplex has been split across the RecC, subunit to create a fork with the separated strands each heading towards, different helicase motor subunits. The strands pass along tunnels within, the complex, both emerging adjacent to the nuclease domain of RecB., Passage of the 3' tail through one of these tunnels provides a mechanism, for the recognition of a Chi sequence by RecC within the context of, double-stranded DNA. Gating of this tunnel suggests how nuclease activity, might be regulated.
About this Structure
1W36 is a Protein complex structure of sequences from Escherichia coli with as ligand. Active as Exodeoxyribonuclease V, with EC number 3.1.11.5 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks., Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB, Nature. 2004 Nov 11;432(7014):187-93. PMID:15538360
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