1w4b
From Proteopedia
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| - | [[Image:1w4b.jpg|left|200px]]<br /><applet load="1w4b" size=" | + | [[Image:1w4b.jpg|left|200px]]<br /><applet load="1w4b" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w4b, resolution 2.3Å" /> | caption="1w4b, resolution 2.3Å" /> | ||
'''P4 PROTEIN FROM PHI12 IN COMPLEX WITH PRODUCT (AMPCPP MG 22C)'''<br /> | '''P4 PROTEIN FROM PHI12 IN COMPLEX WITH PRODUCT (AMPCPP MG 22C)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W4B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi12 Pseudomonas phage phi12] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain C'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1W4B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi12 Pseudomonas phage phi12] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+C'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W4B OCA]. |
==Reference== | ==Reference== | ||
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[[Category: virus dsrna]] | [[Category: virus dsrna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:19:11 2008'' |
Revision as of 08:19, 3 February 2008
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P4 PROTEIN FROM PHI12 IN COMPLEX WITH PRODUCT (AMPCPP MG 22C)
Overview
Many viruses package their genome into preformed capsids using packaging, motors powered by the hydrolysis of ATP. The hexameric ATPase P4 of dsRNA, bacteriophage phi12, located at the vertices of the icosahedral capsid, is, such a packaging motor. We have captured crystallographic structures of P4, for all the key points along the catalytic pathway, including apo, substrate analog bound, and product bound. Substrate and product binding, have been observed as both binary complexes and ternary complexes with, divalent cations. These structures reveal large movements of the putative, RNA binding loop, which are coupled with nucleotide binding and, hydrolysis, indicating how ATP hydrolysis drives RNA translocation through, cooperative conformational changes. Two distinct conformations of bound, nucleotide triphosphate suggest how hydrolysis is activated by RNA, binding. This provides a model for chemomechanical coupling for a, prototype of the large family of hexameric helicases and oligonucleotide, translocating enzymes.
About this Structure
1W4B is a Single protein structure of sequence from Pseudomonas phage phi12 with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Atomic snapshots of an RNA packaging motor reveal conformational changes linking ATP hydrolysis to RNA translocation., Mancini EJ, Kainov DE, Grimes JM, Tuma R, Bamford DH, Stuart DI, Cell. 2004 Sep 17;118(6):743-55. PMID:15369673
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