1w5t
From Proteopedia
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| - | [[Image:1w5t.gif|left|200px]]<br /><applet load="1w5t" size=" | + | [[Image:1w5t.gif|left|200px]]<br /><applet load="1w5t" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w5t, resolution 2.40Å" /> | caption="1w5t, resolution 2.40Å" /> | ||
'''STRUCTURE OF THE AEROPYRUM PERNIX ORC2 PROTEIN (ADPNP-ADP COMPLEXES)'''<br /> | '''STRUCTURE OF THE AEROPYRUM PERNIX ORC2 PROTEIN (ADPNP-ADP COMPLEXES)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix] with MG, ANP and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain C'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1W5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ANP:'>ANP</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+C'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5T OCA]. |
==Reference== | ==Reference== | ||
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[[Category: orc]] | [[Category: orc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:19:39 2008'' |
Revision as of 08:19, 3 February 2008
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STRUCTURE OF THE AEROPYRUM PERNIX ORC2 PROTEIN (ADPNP-ADP COMPLEXES)
Overview
Archaea contain one or more proteins with homology to eukaryotic ORC/Cdc6, proteins. Sequence analysis suggests the existence of at least two, subfamilies of these proteins, for which we propose the nomenclature ORC1, and ORC2. We have determined crystal structures of the ORC2 protein from, the archaeon Aeropyrum pernix in complexes with ADP or a non-hydrolysable, ATP analogue, ADPNP. Between two crystal forms, there are three, crystallographically independent views of the ADP complex and two of the, ADPNP complex. The protein molecules in the three complexes with ADP adopt, very different conformations, while the two complexes with ADPNP are the, same. These structures indicate that there is considerable conformational, flexibility in ORC2 but that ATP binding stabilises a single conformation., We show that the ORC2 protein can bind DNA, and that this activity is, associated with the C-terminal domain of the protein. We present a model, for the interaction of the winged helix (WH) domain of ORC2 with DNA that, differs from that proposed previously for Pyrobaculum aerophilum ORC/Cdc6.
About this Structure
1W5T is a Single protein structure of sequence from Aeropyrum pernix with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Conformational changes induced by nucleotide binding in Cdc6/ORC from Aeropyrum pernix., Singleton MR, Morales R, Grainge I, Cook N, Isupov MN, Wigley DB, J Mol Biol. 2004 Oct 22;343(3):547-57. PMID:15465044
Page seeded by OCA on Sun Feb 3 10:19:39 2008
Categories: Aeropyrum pernix | Single protein | Cook, N. | Grainge, I. | Isupov, M.N. | Morales, R. | Singleton, M.R. | Wigley, D.B. | ADP | ANP | MG | Aaa+ atpase | Cdc6 | Dna binding protein | Dna replication initiation | Orc
