1w6t
From Proteopedia
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| - | [[Image:1w6t.gif|left|200px]]<br /><applet load="1w6t" size=" | + | [[Image:1w6t.gif|left|200px]]<br /><applet load="1w6t" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w6t, resolution 2.10Å" /> | caption="1w6t, resolution 2.10Å" /> | ||
'''CRYSTAL STRUCTURE OF OCTAMERIC ENOLASE FROM STREPTOCOCCUS PNEUMONIAE'''<br /> | '''CRYSTAL STRUCTURE OF OCTAMERIC ENOLASE FROM STREPTOCOCCUS PNEUMONIAE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with MG and 2PE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] Known structural/functional Site: <scene name='pdbsite=AC1:2pe Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1W6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=2PE:'>2PE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] Known structural/functional Site: <scene name='pdbsite=AC1:2pe+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W6T OCA]. |
==Reference== | ==Reference== | ||
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[[Category: surface protein]] | [[Category: surface protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:20:01 2008'' |
Revision as of 08:20, 3 February 2008
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CRYSTAL STRUCTURE OF OCTAMERIC ENOLASE FROM STREPTOCOCCUS PNEUMONIAE
Overview
Alpha-enolases are ubiquitous cytoplasmic, glycolytic enzymes. In, pathogenic bacteria, alpha-enolase doubles as a surface-displayed, plasmin(ogen)-binder supporting virulence. The plasmin(ogen)-binding site, was initially traced to the two C-terminal lysine residues. More recently, an internal nine-amino acid motif comprising residues 248 to 256 was, identified with this function. We report the crystal structure of, alpha-enolase from Streptococcus pneumoniae at 2.0A resolution, the first, structure both of a plasminogen-binding and of an octameric alpha-enolase., While the dimer is structurally similar to other alpha-enolases, the, octamer places the C-terminal lysine residues in an inaccessible, inter-dimer groove restricting the C-terminal lysine residues to a role in, folding and oligomerization. The nine residue plasminogen-binding motif, by contrast, is exposed on the octamer surface revealing this as the, primary site of interaction between alpha-enolase and plasminogen.
About this Structure
1W6T is a Single protein structure of sequence from Streptococcus pneumoniae with and as ligands. Active as Phosphopyruvate hydratase, with EC number 4.2.1.11 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Plasmin(ogen)-binding alpha-enolase from Streptococcus pneumoniae: crystal structure and evaluation of plasmin(ogen)-binding sites., Ehinger S, Schubert WD, Bergmann S, Hammerschmidt S, Heinz DW, J Mol Biol. 2004 Oct 29;343(4):997-1005. PMID:15476816
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