1bs4
From Proteopedia
(New page: 200px<br /> <applet load="1bs4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bs4, resolution 1.90Å" /> '''PEPTIDE DEFORMYLASE...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1BS4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with SO4, ZN and 2PE as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.31 3.5.1.31]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BS4 OCA]]. | + | 1BS4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with SO4, ZN and 2PE as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Formylmethionine_deformylase Formylmethionine deformylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.31 3.5.1.31]]. Structure known Active Sites: ZNA, ZNB and ZNC. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BS4 OCA]]. |
==Reference== | ==Reference== | ||
Iron center, substrate recognition and mechanism of peptide deformylase., Becker A, Schlichting I, Kabsch W, Groche D, Schultz S, Wagner AF, Nat Struct Biol. 1998 Dec;5(12):1053-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9846875 9846875] | Iron center, substrate recognition and mechanism of peptide deformylase., Becker A, Schlichting I, Kabsch W, Groche D, Schultz S, Wagner AF, Nat Struct Biol. 1998 Dec;5(12):1053-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9846875 9846875] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| + | [[Category: Formylmethionine deformylase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Becker, A.]] | [[Category: Becker, A.]] | ||
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[[Category: protein synthesis]] | [[Category: protein synthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:40:19 2007'' |
Revision as of 10:35, 30 October 2007
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PEPTIDE DEFORMYLASE AS ZN2+ CONTAINING FORM (NATIVE) IN COMPLEX WITH INHIBITOR POLYETHYLENE GLYCOL
Overview
Eubacterial proteins are synthesized with a formyl group at the N-terminus, which is hydrolytically removed from the nascent chain by the mononuclear, iron enzyme peptide deformylase. Catalytic efficiency strongly depends on, the identity of the bound metal. We have determined by X-ray, crystallography the Fe2+, Ni2+ and Zn2+ forms of the Escherichia coli, enzyme and a structure in complex with the reaction product Met-Ala-Ser., The structure of the complex, with the tripeptide bound at the active, site, suggests detailed models for the mechanism of substrate recognition, and catalysis. Differences of the protein structures due to the identity, of the bound metal are extremely small and account only for the, observation that Zn2+ binds more tightly than Fe2+ or Ni2+. The striking, loss of ... [(full description)]
About this Structure
1BS4 is a [Single protein] structure of sequence from [Escherichia coli] with SO4, ZN and 2PE as [ligands]. Active as [Formylmethionine deformylase], with EC number [3.5.1.31]. Structure known Active Sites: ZNA, ZNB and ZNC. Full crystallographic information is available from [OCA].
Reference
Iron center, substrate recognition and mechanism of peptide deformylase., Becker A, Schlichting I, Kabsch W, Groche D, Schultz S, Wagner AF, Nat Struct Biol. 1998 Dec;5(12):1053-8. PMID:9846875
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