1w8j
From Proteopedia
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| - | [[Image:1w8j.gif|left|200px]]<br /><applet load="1w8j" size=" | + | [[Image:1w8j.gif|left|200px]]<br /><applet load="1w8j" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w8j, resolution 2.7Å" /> | caption="1w8j, resolution 2.7Å" /> | ||
'''CRYSTAL STRUCTURE OF MYOSIN V MOTOR DOMAIN-NUCLEOTIDE-FREE'''<br /> | '''CRYSTAL STRUCTURE OF MYOSIN V MOTOR DOMAIN-NUCLEOTIDE-FREE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W8J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain D'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1W8J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+D'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W8J OCA]. |
==Reference== | ==Reference== | ||
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[[Category: unconventional myosin]] | [[Category: unconventional myosin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:20:34 2008'' |
Revision as of 08:20, 3 February 2008
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CRYSTAL STRUCTURE OF MYOSIN V MOTOR DOMAIN-NUCLEOTIDE-FREE
Overview
The molecular motor, myosin, undergoes conformational changes in order to, convert chemical energy into force production. Based on kinetic and, structural considerations, we assert that three crystal forms of the, myosin V motor delineate the conformational changes that myosin motors, undergo upon detachment from actin. First, a motor domain structure, demonstrates that nucleotide-free myosin V adopts a specific state, (rigor-like) that is not influenced by crystal packing. A second structure, reveals an actomyosin state that favors rapid release of ADP, and differs, from the rigor-like state by a P-loop rearrangement. Comparison of these, structures with a third structure, a 2.0 angstroms resolution structure of, the motor bound to an ATP analog, illuminates the structural features that, provide communication between the actin interface and nucleotide-binding, site. Paramount among these is a region we name the transducer, which is, composed of the seven-stranded beta-sheet and associated loops and, linkers. Reminiscent of the beta-sheet distortion of the F1-ATPase, sequential distortion of this transducer region likely controls sequential, release of products from the nucleotide pocket during force generation.
About this Structure
1W8J is a Single protein structure of sequence from Gallus gallus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Three myosin V structures delineate essential features of chemo-mechanical transduction., Coureux PD, Sweeney HL, Houdusse A, EMBO J. 2004 Nov 24;23(23):4527-37. Epub 2004 Oct 28. PMID:15510214
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