1bs0
From Proteopedia
(New page: 200px<br /> <applet load="1bs0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bs0, resolution 1.65Å" /> '''PLP-DEPENDENT ACYL-...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1BS0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.47 2.3.1.47]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BS0 OCA]]. | + | 1BS0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/8-amino-7-oxononanoate_synthase 8-amino-7-oxononanoate synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.47 2.3.1.47]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BS0 OCA]]. |
==Reference== | ==Reference== | ||
The crystal structure of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl-CoA-condensing enzyme., Alexeev D, Alexeeva M, Baxter RL, Campopiano DJ, Webster SP, Sawyer L, J Mol Biol. 1998 Nov 27;284(2):401-19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9813126 9813126] | The crystal structure of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl-CoA-condensing enzyme., Alexeev D, Alexeeva M, Baxter RL, Campopiano DJ, Webster SP, Sawyer L, J Mol Biol. 1998 Nov 27;284(2):401-19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9813126 9813126] | ||
| + | [[Category: 8-amino-7-oxononanoate synthase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: plp-dependent acyl-coa synthase]] | [[Category: plp-dependent acyl-coa synthase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:40:27 2007'' |
Revision as of 10:35, 30 October 2007
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PLP-DEPENDENT ACYL-COA SYNTHASE
Overview
8-Amino-7-oxononanoate synthase (or 8-amino-7-ketopelargonate synthase; EC, 2.3.1.47; AONS) catalyses the decarboxylative condensation of l-alanine, and pimeloyl-CoA in the first committed step of biotin biosynthesis. We, have cloned, over-expressed and purified AONS from Escherichia coli and, determined the crystal structures of the apo and PLP-bound forms of the, enzyme. The protein is a symmetrical homodimer with a tertiary structure, and active site organisation similar to, but distinct from, those of other, PLP-dependent enzymes whose three-dimensional structures are known. The, critical PLP-binding lysine of AONS is located at the end of a deep cleft, that allows access of the pantothenate arm of pimeloyl-CoA. A cluster of, positively charged residues at the entrance to this cleft ... [(full description)]
About this Structure
1BS0 is a [Single protein] structure of sequence from [Escherichia coli] with SO4 as [ligand]. Active as [8-amino-7-oxononanoate synthase], with EC number [2.3.1.47]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
Reference
The crystal structure of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl-CoA-condensing enzyme., Alexeev D, Alexeeva M, Baxter RL, Campopiano DJ, Webster SP, Sawyer L, J Mol Biol. 1998 Nov 27;284(2):401-19. PMID:9813126
Page seeded by OCA on Tue Oct 30 12:40:27 2007
Categories: 8-amino-7-oxononanoate synthase | Escherichia coli | Single protein | Alexeev, D. | Alexeeva, M. | Baxter, R.L. | Campopiano, D.J. | Sawyer, L. | Webster, S.P. | SO4 | 8-amino-7-ketopelargonate synthase | 8-amino-7-oxonanoate synthase | Biotin biosynthesis | Plp-dependent acyl-coa synthase
