2bsw
From Proteopedia
(New page: 200px<br /> <applet load="2bsw" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bsw, resolution 1.63Å" /> '''CRYSTAL STRUCTURE O...) |
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==About this Structure== | ==About this Structure== | ||
- | 2BSW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ ]] with SO4, CAO and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BSW OCA]]. | + | 2BSW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ ]] with SO4, CAO and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BSW OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
- | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:40:32 2007'' |
Revision as of 10:35, 30 October 2007
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CRYSTAL STRUCTURE OF A GLYPHOSATE-N-ACETYLTRANSFERASE OBTAINED BY DNA SHUFFLING.
Overview
The success of structural studies performed on an individual target in, small scale or on many targets in the system-wide scale of structural, genomics depends critically on three parameters: (i) obtaining an, expression system capable of producing large quantities of the, macromolecule(s) of interest, (ii) purifying this material in soluble, form, and (iii) obtaining diffraction-quality crystals suitable for x-ray, analysis. The attrition rate caused by these constraints is often quite, high. Here, we present a strategy that addresses each of these three, parameters simultaneously. Using DNA shuffling to introduce functional, sequence variability into a protein of interest, we screened crude lysate, supernatants for soluble variants that retain enzymatic activity., Crystallization trials ... [(full description)]
About this Structure
2BSW is a [Single protein] structure of sequence from [[1]] with SO4, CAO and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
DNA shuffling as a tool for protein crystallization., Keenan RJ, Siehl DL, Gorton R, Castle LA, Proc Natl Acad Sci U S A. 2005 Jun 21;102(25):8887-92. Epub 2005 Jun 10. PMID:15951425 [[Category: ]]
Page seeded by OCA on Tue Oct 30 12:40:32 2007