1znm
From Proteopedia
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| - | [[Image:1znm.gif|left|200px]]<br /><applet load="1znm" size=" | + | [[Image:1znm.gif|left|200px]]<br /><applet load="1znm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1znm" /> | caption="1znm" /> | ||
'''A ZINC FINGER WITH AN ARTIFICIAL BETA-TURN, ORIGINAL SEQUENCE TAKEN FROM THE THIRD ZINC FINGER DOMAIN OF THE HUMAN TRANSCRIPTIONAL REPRESSOR PROTEIN YY1 (YING AND YANG 1, A DELTA TRANSCRIPTION FACTOR), NMR, 34 STRUCTURES'''<br /> | '''A ZINC FINGER WITH AN ARTIFICIAL BETA-TURN, ORIGINAL SEQUENCE TAKEN FROM THE THIRD ZINC FINGER DOMAIN OF THE HUMAN TRANSCRIPTIONAL REPRESSOR PROTEIN YY1 (YING AND YANG 1, A DELTA TRANSCRIPTION FACTOR), NMR, 34 STRUCTURES'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZNM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=BTD:Beta Turn Dipeptide (Type II' Beta Turn Mimetic)'>BTD</scene> and <scene name='pdbsite=ZNB:Zn Binding Site'>ZNB</scene>. Full crystallographic information is available from [http:// | + | 1ZNM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=BTD:Beta+Turn+Dipeptide+(Type+II'+Beta+Turn+Mimetic)'>BTD</scene> and <scene name='pdbsite=ZNB:Zn+Binding+Site'>ZNB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZNM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zn-btd(7]] | [[Category: zn-btd(7]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:22:41 2008'' |
Revision as of 08:22, 3 February 2008
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A ZINC FINGER WITH AN ARTIFICIAL BETA-TURN, ORIGINAL SEQUENCE TAKEN FROM THE THIRD ZINC FINGER DOMAIN OF THE HUMAN TRANSCRIPTIONAL REPRESSOR PROTEIN YY1 (YING AND YANG 1, A DELTA TRANSCRIPTION FACTOR), NMR, 34 STRUCTURES
Overview
We have incorporated a bicyclic beta-turn mimetic (BTD; beta-turn, dipeptide) into a zinc finger, creating a zinc finger with an artificial, beta-turn. The designed peptide chelates zinc and has the same fold as the, unmodified native zinc finger (finger 3 of the human YY1 protein). A, combination of 1H NMR and structure calculations reveals that, in, solution, this zinc finger has a fold similar to the known wild-type, crystal structure and to other zinc fingers containing the consensus, sequence X3-Cys-X4-Cys-X12-His-X3-His-X. The peptide was designed with BTD, between the chelating cysteine residues, with BTD forming a type II', beta-turn linking the two strands of a distorted anti-parallel beta-sheet., The C-terminal portion of the peptide forms a helix with zinc, co-ordinating histidine residues on successive turns of the helix. This, work represents a step towards developing methods by which parts of a, target protein may be replaced by peptide mimetics.
About this Structure
1ZNM is a Single protein structure of sequence from [1] with as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Design, synthesis and structure of a zinc finger with an artificial beta-turn., Viles JH, Patel SU, Mitchell JB, Moody CM, Justice DE, Uppenbrink J, Doyle PM, Harris CJ, Sadler PJ, Thornton JM, J Mol Biol. 1998 Jun 19;279(4):973-86. PMID:9642075
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