2adr
From Proteopedia
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| - | [[Image:2adr.jpg|left|200px]]<br /><applet load="2adr" size=" | + | [[Image:2adr.jpg|left|200px]]<br /><applet load="2adr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2adr" /> | caption="2adr" /> | ||
'''ADR1 DNA-BINDING DOMAIN FROM SACCHAROMYCES CEREVISIAE, NMR, 25 STRUCTURES'''<br /> | '''ADR1 DNA-BINDING DOMAIN FROM SACCHAROMYCES CEREVISIAE, NMR, 25 STRUCTURES'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ADR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=ZNC:Zn Finger 2'>ZNC</scene>. Full crystallographic information is available from [http:// | + | 2ADR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=ZNC:Zn+Finger+2'>ZNC</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:22:53 2008'' |
Revision as of 08:22, 3 February 2008
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ADR1 DNA-BINDING DOMAIN FROM SACCHAROMYCES CEREVISIAE, NMR, 25 STRUCTURES
Overview
The region responsible for sequence-specific DNA binding by the, transcription factor ADR1 contains two Cys2-His2 zinc fingers and an, additional N-terminal proximal accessory region (PAR). The N-terminal, (non-finger) PAR is unstructured in the absence of DNA and undergoes a, folding transition on binding the DNA transcription target site. We have, used a set of HN-HN NOEs derived from a perdeuterated protein-DNA complex, to describe the fold of ADR1 bound to the UAS1 binding site. The PAR forms, a compact domain consisting of three antiparallel strands that contact A-T, base pairs in the major groove. The three-strand domain is a novel fold, among all known DNA-binding proteins. The PAR shares sequence homology, with the N-terminal regions of other zinc finger proteins, suggesting that, it represents a new DNA-binding module that extends the binding repertoire, of zinc finger proteins.
About this Structure
2ADR is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
A folding transition and novel zinc finger accessory domain in the transcription factor ADR1., Bowers PM, Schaufler LE, Klevit RE, Nat Struct Biol. 1999 May;6(5):478-85. PMID:10331877
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