2bgc
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2bgc.gif|left|200px]]<br /><applet load="2bgc" size=" | + | [[Image:2bgc.gif|left|200px]]<br /><applet load="2bgc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bgc, resolution 2.30Å" /> | caption="2bgc, resolution 2.30Å" /> | ||
'''PRFA-G145S, A CONSTITUTIVE ACTIVE MUTANT OF THE TRANSCRIPTIONAL REGULATOR IN L.MONOCYTOGENES'''<br /> | '''PRFA-G145S, A CONSTITUTIVE ACTIVE MUTANT OF THE TRANSCRIPTIONAL REGULATOR IN L.MONOCYTOGENES'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2BGC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes] with DTU and DTT as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Dtt Binding Site For Chain F'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2BGC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes] with <scene name='pdbligand=DTU:'>DTU</scene> and <scene name='pdbligand=DTT:'>DTT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Dtt+Binding+Site+For+Chain+F'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BGC OCA]. |
==Reference== | ==Reference== | ||
| Line 24: | Line 24: | ||
[[Category: transcriptional regulator]] | [[Category: transcriptional regulator]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:23:43 2008'' |
Revision as of 08:23, 3 February 2008
|
PRFA-G145S, A CONSTITUTIVE ACTIVE MUTANT OF THE TRANSCRIPTIONAL REGULATOR IN L.MONOCYTOGENES
Overview
Listeria monocytogenes, a Gram-positive, facultative intracellular human, pathogen, causes systemic infections with high mortality rate. The, majority of the known pathogenicity factors of L. monocytogenes is, regulated by a single transcription factor, PrfA. Hyperhaemolytic, laboratory strains of L. monocytogenes express the constitutively active, mutant PrfA(G145S) inducing virulence gene overexpression independent of, environmental conditions. PrfA belongs to the Crp/Fnr family of, transcription factors generally activated by a small effector, such as, cAMP or O(2). We present the crystal structures of wild-type PrfA, the, first Gram-positive member of the Crp/Fnr family, and of the, constitutively active mutant PrfA(G145S). Cap (Crp) has previously been, described exclusively in the cAMP-induced (DNA-free and -bound), conformation. By contrast, the PrfA structures present views both of the, non-induced state and of the mutationally activated form. The low, DNA-binding affinity of wild-type PrfA is supported both structurally, (partly disordered helix-turn-helix motif, overall geometry of the HTH, alpha-helices deviates from Cap) and by surface plasmon resonance analyses, (K(D) = 0.9 microM). In PrfA(G145S) the HTH motifs dramatically rearrange, to adopt a conformation comparable to cAMP-induced Cap and hence, favourable for DNA binding, supported by a DNA-binding affinity of 50 nM., Finally, the hypothesis that wild-type PrfA, like other Crp/Fnr family, members, may require an as yet unidentified cofactor for activation is, supported by the presence of a distinct tunnel in PrfA, located at the, interface of the beta-barrel and the DNA-binding domain.
About this Structure
2BGC is a Single protein structure of sequence from Listeria monocytogenes with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The mutation G145S in PrfA, a key virulence regulator of Listeria monocytogenes, increases DNA-binding affinity by stabilizing the HTH motif., Eiting M, Hageluken G, Schubert WD, Heinz DW, Mol Microbiol. 2005 Apr;56(2):433-46. PMID:15813735
Page seeded by OCA on Sun Feb 3 10:23:43 2008
