2bir
From Proteopedia
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| - | [[Image:2bir.jpg|left|200px]]<br /><applet load="2bir" size=" | + | [[Image:2bir.jpg|left|200px]]<br /><applet load="2bir" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bir, resolution 2.30Å" /> | caption="2bir, resolution 2.30Å" /> | ||
'''ADDITIVITY OF SUBSTRATE BINDING IN RIBONUCLEASE T1 (Y42A MUTANT)'''<br /> | '''ADDITIVITY OF SUBSTRATE BINDING IN RIBONUCLEASE T1 (Y42A MUTANT)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BIR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae] with CA and 2GP as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=S1:Catalytic Site'>S1</scene>. Full crystallographic information is available from [http:// | + | 2BIR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=2GP:'>2GP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=S1:Catalytic+Site'>S1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BIR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: signal]] | [[Category: signal]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:24:37 2008'' |
Revision as of 08:24, 3 February 2008
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ADDITIVITY OF SUBSTRATE BINDING IN RIBONUCLEASE T1 (Y42A MUTANT)
Overview
It has been established that Tyr-42, Tyr-45, and Glu-46 take part in a, structural motif that renders guanine specificity to ribonuclease T1. We, report on the impact of Tyr-42, Tyr-45, and Glu-46 substitutions on the, guanine specificity of RNase T1. The Y42A and E46A mutations profoundly, affect substrate binding. No such effect is observed for Y45A RNase T1., From the kinetics of the Y42A/Y45A and Y42A/E46A double mutants, we, conclude that these pairs of residues contribute to guanine specificity in, a mutually independent way. From our results, it appears that the, energetic contribution of aromatic face-to-face stacking interactions may, be significant if polycyclic molecules, such as guanine, are involved.
About this Structure
2BIR is a Single protein structure of sequence from Aspergillus oryzae with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Additivity of protein-guanine interactions in ribonuclease T1., Loverix S, Doumen J, Steyaert J, J Biol Chem. 1997 Apr 11;272(15):9635-9. PMID:9092491
Page seeded by OCA on Sun Feb 3 10:24:37 2008
Categories: Aspergillus oryzae | Single protein | Doumen, J. | Loverix, S. | Steyaert, J. | 2GP | CA | Endonuclease | Hydrolase | Nuclease | Ribonuclease | Signal
