2bj8
From Proteopedia
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| - | [[Image:2bj8.gif|left|200px]]<br /><applet load="2bj8" size=" | + | [[Image:2bj8.gif|left|200px]]<br /><applet load="2bj8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bj8, resolution 2.10Å" /> | caption="2bj8, resolution 2.10Å" /> | ||
'''NIKR IN CLOSED CONFORMATION AND NICKEL BOUND TO HIGH AND LOW-AFFINITY SITES'''<br /> | '''NIKR IN CLOSED CONFORMATION AND NICKEL BOUND TO HIGH AND LOW-AFFINITY SITES'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BJ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with NI, CL, PG4, EPE and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Epe Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2BJ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=PG4:'>PG4</scene>, <scene name='pdbligand=EPE:'>EPE</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Epe+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJ8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:24:47 2008'' |
Revision as of 08:24, 3 February 2008
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NIKR IN CLOSED CONFORMATION AND NICKEL BOUND TO HIGH AND LOW-AFFINITY SITES
Overview
The Pyrococcus horikoshii OT3 genome contains a gene (PH0601 or nikR), encoding a protein (PhNikR) that shares 33.8% amino acid sequence identity, with Escherichia coli nickel responsive repressor NikR (EcNikR), including, many residues that are functionally important in the E.coli ortholog. We, succeeded in crystallization and structural characterization of PhNikR in, the apo form and two nickel bound forms that exhibit different, conformations, open and closed. Moreover, we have identified a putative, "low-affinity" nickel-binding pocket in the closed form. This binding site, has unusual nickel coordination and exhibits high sensitivity to phosphate, in the crystal structure. Analysis of the PhNikR structures and, structure-based mutational studies with EcNikR reveals a plausible, mechanism of nickel-dependent promoter recognition by the NikR family of, proteins.
About this Structure
2BJ8 is a Single protein structure of sequence from Pyrococcus horikoshii with , , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of Pyrococcus horikoshii NikR: nickel sensing and implications for the regulation of DNA recognition., Chivers PT, Tahirov TH, J Mol Biol. 2005 May 6;348(3):597-607. PMID:15826657
Page seeded by OCA on Sun Feb 3 10:24:47 2008
Categories: Pyrococcus horikoshii | Single protein | RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative. | Tahirov, T.H. | CL | EDO | EPE | NI | PG4 | Dna-binding | Metal-binding | Nickel | Nikr | Repressor | Riken structural genomics/proteomics initiative | Rsgi | Structural genomics | Transcription | Transcription regulation
