2bjr
From Proteopedia
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| - | [[Image:2bjr.gif|left|200px]]<br /><applet load="2bjr" size=" | + | [[Image:2bjr.gif|left|200px]]<br /><applet load="2bjr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bjr, resolution 1.80Å" /> | caption="2bjr, resolution 1.80Å" /> | ||
'''CRYSTAL STRUCTURE OF THE NEMATODE SPERM CELL MOTILITY PROTEIN MFP2B'''<br /> | '''CRYSTAL STRUCTURE OF THE NEMATODE SPERM CELL MOTILITY PROTEIN MFP2B'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BJR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ascaris_suum Ascaris suum] with ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2BJR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ascaris_suum Ascaris suum] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: nematode]] | [[Category: nematode]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:24:58 2008'' |
Revision as of 08:24, 3 February 2008
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CRYSTAL STRUCTURE OF THE NEMATODE SPERM CELL MOTILITY PROTEIN MFP2B
Overview
The simplicity and specialization of the cell motility machinery of, Ascaris sperm provides a powerful system in which to probe the basic, molecular mechanism of amoeboid cell motility. Although Ascaris sperm, locomotion closely resembles that seen in many other types of crawling, cell, movement is generated by modulation of a cytoskeleton based on the, major sperm protein (MSP) rather than the actin present in other cell, types. The Ascaris motility machinery can be studied conveniently in a, cell-free in vitro system based on the movement of plasma membrane, vesicles by fibres constructed from bundles of MSP filaments. In addition, to ATP, MSP and a plasma membrane protein, reconstitution of MSP motility, in this cell-free extract requires cytosolic proteins to orchestrate the, site-specific assembly and bundling of MSP filaments that generates, locomotion. One of these proteins, MFP2, accelerates the rate of movement, in this assay. Here, we describe crystal structures of two isoforms of, MFP2 and show that both are constructed from two domains that have the, same fold based on a novel, compact beta sheet arrangement. Patterns of, conservation observed in a structure-based analysis of MFP2 sequences from, different nematode species identified regions that may be putative, functional interfaces involved both in interactions between MFP2 domains, and also with other components of the sperm motility machinery. Analysis, of the growth of fibres in vitro in the presence of added MFP2 indicated, that MFP2 increases the rate of locomotion by enhancing the effective rate, of MSP filament polymerization. This observation, together with the, structural data, suggests that MFP2 may function in a manner analogous to, formins in actin-based motility.
About this Structure
2BJR is a Single protein structure of sequence from Ascaris suum with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of MFP2 and its function in enhancing MSP polymerization in Ascaris sperm amoeboid motility., Grant RP, Buttery SM, Ekman GC, Roberts TM, Stewart M, J Mol Biol. 2005 Apr 1;347(3):583-95. PMID:15755452
Page seeded by OCA on Sun Feb 3 10:24:58 2008
Categories: Ascaris suum | Single protein | Buttery, S.M. | Ekman, G.C. | Grant, R.P. | Roberts, T.M. | Stewart, M. | SO4 | ZN | Ascaris | Motility | Msp | Nematode
