1h4h
From Proteopedia
(New page: 200px<br /> <applet load="1h4h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h4h, resolution 1.90Å" /> '''OLIGOSACCHARIDE-BIN...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1H4H is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_agaradhaerens Bacillus agaradhaerens]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H4H OCA]]. | + | 1H4H is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_agaradhaerens Bacillus agaradhaerens]]. Active as [[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Structure known Active Site: BC3. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H4H OCA]]. |
==Reference== | ==Reference== | ||
Oligosaccharide binding to family 11 xylanases: both covalent intermediate and mutant product complexes display (2,5)B conformations at the active centre., Sabini E, Wilson KS, Danielsen S, Schulein M, Davies GJ, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1344-7. Epub 2001, Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11526340 11526340] | Oligosaccharide binding to family 11 xylanases: both covalent intermediate and mutant product complexes display (2,5)B conformations at the active centre., Sabini E, Wilson KS, Danielsen S, Schulein M, Davies GJ, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1344-7. Epub 2001, Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11526340 11526340] | ||
[[Category: Bacillus agaradhaerens]] | [[Category: Bacillus agaradhaerens]] | ||
| + | [[Category: Endo-1,4-beta-xylanase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Danielsen, S.]] | [[Category: Danielsen, S.]] | ||
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[[Category: xylanase]] | [[Category: xylanase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:42:17 2007'' |
Revision as of 10:37, 30 October 2007
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OLIGOSACCHARIDE-BINDING TO FAMILY 11 XYLANASES: BOTH COVALENT INTERMEDIATE AND MUTANT-PRODUCT COMPLEXES DISPLAY 2,5B CONFORMATIONS AT THE ACTIVE-CENTRE
Overview
The glycoside hydrolase sequence-based classification reveals two families, of enzymes which hydrolyse the beta-1,4-linked backbone of xylan, xylanases, termed families GH-10 and GH-11. Family GH-11 xylanases are, intriguing in that catalysis is performed via a covalent intermediate, adopting an unusual (2,5)B (boat) conformation, a conformation which also, fulfils the stereochemical constraints of the oxocarbenium ion-like, transition state. Here, the 1.9 A structure of a nucleophile, E94A, mutant, of the Xyn11 from Bacillus agaradhaerens in complex with xylotriose is, presented. Intriguingly, this complex also adopts the (2,5)B conformation, in the -1 subsite, with the vacant space provided by the Glu-->Ala, mutation allowing the sugar to adopt the alpha-configuration at C1. The, ... [(full description)]
About this Structure
1H4H is a [Single protein] structure of sequence from [Bacillus agaradhaerens]. Active as [Endo-1,4-beta-xylanase], with EC number [3.2.1.8]. Structure known Active Site: BC3. Full crystallographic information is available from [OCA].
Reference
Oligosaccharide binding to family 11 xylanases: both covalent intermediate and mutant product complexes display (2,5)B conformations at the active centre., Sabini E, Wilson KS, Danielsen S, Schulein M, Davies GJ, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1344-7. Epub 2001, Aug 23. PMID:11526340
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