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1h46
From Proteopedia
(New page: 200px<br /> <applet load="1h46" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h46, resolution 1.52Å" /> '''THE CATALYTIC MODUL...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1H46 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium]] with NAG and RNP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H46 OCA]]. | + | 1H46 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium]] with NAG and RNP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91]]. Structure known Active Site: NAG. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H46 OCA]]. |
==Reference== | ==Reference== | ||
The catalytic module of Cel7D from Phanerochaete chrysosporium as a chiral selector: structural studies of its complex with the beta blocker (R)-propranolol., Munoz IG, Mowbray SL, Stahlberg J, Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):637-43. Epub 2003, Mar 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12657782 12657782] | The catalytic module of Cel7D from Phanerochaete chrysosporium as a chiral selector: structural studies of its complex with the beta blocker (R)-propranolol., Munoz IG, Mowbray SL, Stahlberg J, Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):637-43. Epub 2003, Mar 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12657782 12657782] | ||
| + | [[Category: Cellulose 1,4-beta-cellobiosidase]] | ||
[[Category: Phanerochaete chrysosporium]] | [[Category: Phanerochaete chrysosporium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: glycoside hydrolase]] | [[Category: glycoside hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:42:36 2007'' |
Revision as of 10:37, 30 October 2007
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THE CATALYTIC MODULE OF CEL7D FROM PHANEROCHAETE CHRYSOSPORIUM AS A CHIRAL SELECTOR: STRUCTURAL STUDIES OF ITS COMPLEX WITH THE B-BLOCKER (R)-PROPRANOLOL
Overview
Previous investigations have shown that the major cellobiohydrolase of, Phanerochaete chrysosporium, Cel7D (CBH 58), can be used to separate the, enantiomers of a number of drugs, including adrenergic beta blockers such, as propranolol. The structural basis of this enantioselectivity is, explored here. A 1.5 A X-ray structure of the catalytic domain of Cel7D in, complex with (R)-propranolol showed the ligand bound at the active site in, glucosyl-binding subsites -1/+1. The catalytic residue Glu207 makes a, strong charge-charge interaction with the secondary amine of, (R)-propranolol; this is supported by a second interaction of the amine, with the nearby Asp209. The aromatic naphthyl group stacks onto the indole, ring of Trp373 (normally the glucosyl-binding platform of subsite +1)., ... [(full description)]
About this Structure
1H46 is a [Single protein] structure of sequence from [Phanerochaete chrysosporium] with NAG and RNP as [ligands]. Active as [Cellulose 1,4-beta-cellobiosidase], with EC number [3.2.1.91]. Structure known Active Site: NAG. Full crystallographic information is available from [OCA].
Reference
The catalytic module of Cel7D from Phanerochaete chrysosporium as a chiral selector: structural studies of its complex with the beta blocker (R)-propranolol., Munoz IG, Mowbray SL, Stahlberg J, Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):637-43. Epub 2003, Mar 25. PMID:12657782
Page seeded by OCA on Tue Oct 30 12:42:36 2007
