2br3
From Proteopedia
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| - | [[Image:2br3.gif|left|200px]]<br /><applet load="2br3" size=" | + | [[Image:2br3.gif|left|200px]]<br /><applet load="2br3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2br3, resolution 2.79Å" /> | caption="2br3, resolution 2.79Å" /> | ||
'''CMCI-D160 MG'''<br /> | '''CMCI-D160 MG'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BR3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with MG, 15P and PEG as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain F'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2BR3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=15P:'>15P</scene> and <scene name='pdbligand=PEG:'>PEG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+F'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: porin]] | [[Category: porin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:26:55 2008'' |
Revision as of 08:26, 3 February 2008
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CMCI-D160 MG
Overview
Cephamycin C-producing microorganisms use two enzymes to convert, cephalosporins to their 7alpha-methoxy derivatives. Here we report the, X-ray structure of one of these enzymes, CmcI, from Streptomyces, clavuligerus. The polypeptide chain of the enzyme folds into a C-terminal, Rossmann domain and a smaller N-terminal domain, and the molecule packs as, a hexamer in the crystal. The Rossmann domain binds, S-adenosyl-L-methionine (SAM) and the demethylated product, S-adenosyl-L-homocysteine, in a fashion similar to the common binding mode, of this cofactor in SAM-dependent methyltransferases. There is a, magnesium-binding site in the vicinity of the SAM site with a bound, magnesium ion ligated by residues Asp160, Glu186 and Asp187. The expected, cephalosporin binding site near the magnesium ion is occupied by, polyethyleneglycol (PEG) from the crystallisation medium. The geometry of, the SAM and the magnesium binding sites is similar to that found in, cathechol O-methyltransferase. The results suggest CmcI is a, methyltransferase, and its most likely function is to catalyse the, transfer of a methyl group from SAM to the 7alpha-hydroxy cephalosporin in, the second catalytic reaction of cephamycin formation. Based on the, docking of the putative substrate, 7alpha-hydroxy-O-carbamoyldeacetylcephalosporin C, to the structure of the, ternary CmcI-Mg2+-SAM complex, we propose a model for substrate binding, and catalysis. In this model, the 7-hydroxy group of the beta-lactam ring, ligates the Mg2+ with its alpha-side facing the methyl group of SAM at a, distance that would allow methylation of the hydroxyl-group.
About this Structure
2BR3 is a Single protein structure of sequence from Streptomyces clavuligerus with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Insights into cephamycin biosynthesis: the crystal structure of CmcI from Streptomyces clavuligerus., Oster LM, Lester DR, Terwisscha van Scheltinga A, Svenda M, van Lun M, Genereux C, Andersson I, J Mol Biol. 2006 Apr 28;358(2):546-58. Epub 2006 Feb 21. PMID:16527306
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