2bra
From Proteopedia
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| - | [[Image:2bra.gif|left|200px]]<br /><applet load="2bra" size=" | + | [[Image:2bra.gif|left|200px]]<br /><applet load="2bra" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bra, resolution 2.00Å" /> | caption="2bra, resolution 2.00Å" /> | ||
'''STRUCTURE OF N-TERMINAL FAD BINDING MOTIF OF MOUSE MICAL'''<br /> | '''STRUCTURE OF N-TERMINAL FAD BINDING MOTIF OF MOUSE MICAL'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BRA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with CL and FAD as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cl Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2BRA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BRA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:27:00 2008'' |
Revision as of 08:27, 3 February 2008
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STRUCTURE OF N-TERMINAL FAD BINDING MOTIF OF MOUSE MICAL
Overview
During development, neurons are guided to their targets by short- and, long-range attractive and repulsive cues. MICAL, a large multidomain, protein, is required for the combined action of semaphorins and plexins in, axon guidance. Here, we present the structure of the N-terminal region of, MICAL (MICAL(fd)) determined by x-ray diffraction to 2.0 A resolution. The, structure shows that MICAL(fd) is an FAD-containing module structurally, similar to aromatic hydroxylases and amine oxidases. In addition, we, present biochemical data that show that MICAL(fd) is a flavoenzyme that in, the presence of NADPH reduces molecular oxygen to H(2)O(2) (K(m,NAPDH) =, 222 microM; k(cat) = 77 sec(-1)), a molecule with known signaling, properties. We propose that the H(2)O(2) produced by this reaction may be, one of the signaling molecules involved in axon guidance by MICAL.
About this Structure
2BRA is a Single protein structure of sequence from Mus musculus with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure and activity of the axon guidance protein MICAL., Nadella M, Bianchet MA, Gabelli SB, Barrila J, Amzel LM, Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16830-5. Epub 2005 Nov 7. PMID:16275926
Page seeded by OCA on Sun Feb 3 10:27:00 2008
Categories: Mus musculus | Single protein | Amzel, L.M. | Bianchet, M.A. | Gabelli, S.B. | Nadella, M. | CL | FAD | Axon guidance | Coiled coil | Cytoskeleton | Fad | Flavoprotein | Lim domain | Metal-binding | Plexin | Redox | Vesicle transport | Zinc
