2bt7
From Proteopedia
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| - | [[Image:2bt7.gif|left|200px]]<br /><applet load="2bt7" size=" | + | [[Image:2bt7.gif|left|200px]]<br /><applet load="2bt7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bt7, resolution 2.35Å" /> | caption="2bt7, resolution 2.35Å" /> | ||
'''STRUCTURE OF THE C-TERMINAL RECEPTOR-BINDING DOMAIN OF AVIAN REOVIRUS FIBRE SIGMAC, CD CRYSTAL FORM'''<br /> | '''STRUCTURE OF THE C-TERMINAL RECEPTOR-BINDING DOMAIN OF AVIAN REOVIRUS FIBRE SIGMAC, CD CRYSTAL FORM'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BT7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Avian_orthoreovirus Avian orthoreovirus] with SO4 and CD as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cdm Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2BT7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Avian_orthoreovirus Avian orthoreovirus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cdm+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BT7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: viral protein]] | [[Category: viral protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:27:35 2008'' |
Revision as of 08:27, 3 February 2008
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STRUCTURE OF THE C-TERMINAL RECEPTOR-BINDING DOMAIN OF AVIAN REOVIRUS FIBRE SIGMAC, CD CRYSTAL FORM
Overview
Avian reovirus fibre, a homo-trimer of the sigmaC protein, is responsible, for primary host cell attachment. The protein expressed in bacteria forms, elongated fibres comprised of a carboxy-terminal globular head domain and, a slender shaft, and partial proteolysis yielded a carboxy-terminal, protease-stable domain that was amenable to crystallisation. Here, we show, that this fragment retains receptor-binding capability and report its, structure, solved using two-wavelength anomalous diffraction and refined, using data collected from three different crystal forms at 2.1 angstroms, 2.35 angstroms and 3.0 angstroms resolution. The carboxy-terminal globular, domain has a beta-barrel fold with the same overall topology as the, mammalian reovirus fibre (sigma1). However, the monomers of the sigmaC, trimer show a more splayed-out arrangement than in the sigma1 structure., Also resolved are two triple beta-spiral repeats of the shaft or stalk, domain. The presence in the sequence of heptad repeats amino-terminal to, these triple beta-spiral repeats suggests that the unresolved portion of, the shaft domain contains a triple alpha-helical coiled-coil structure., Implications for the function and stability of the sigmaC protein are, discussed.
About this Structure
2BT7 is a Single protein structure of sequence from Avian orthoreovirus with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of the carboxy-terminal receptor-binding domain of avian reovirus fibre sigmaC., Guardado Calvo P, Fox GC, Hermo Parrado XL, Llamas-Saiz AL, Costas C, Martinez-Costas J, Benavente J, van Raaij MJ, J Mol Biol. 2005 Nov 18;354(1):137-49. Epub 2005 Sep 30. PMID:16236316
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