2bvt
From Proteopedia
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| - | [[Image:2bvt.gif|left|200px]]<br /><applet load="2bvt" size=" | + | [[Image:2bvt.gif|left|200px]]<br /><applet load="2bvt" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bvt, resolution 2.90Å" /> | caption="2bvt, resolution 2.90Å" /> | ||
'''THE STRUCTURE OF A MODULAR ENDO-BETA-1,4-MANNANASE FROM CELLULOMONAS FIMI EXPLAINS THE PRODUCT SPECIFICITY OF GLYCOSIDE HYDROLASE FAMILY 26 MANNANASES.'''<br /> | '''THE STRUCTURE OF A MODULAR ENDO-BETA-1,4-MANNANASE FROM CELLULOMONAS FIMI EXPLAINS THE PRODUCT SPECIFICITY OF GLYCOSIDE HYDROLASE FAMILY 26 MANNANASES.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BVT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cellulomonas_fimi Cellulomonas fimi] with CAC as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] Known structural/functional Site: <scene name='pdbsite=AC1:Cac Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2BVT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cellulomonas_fimi Cellulomonas fimi] with <scene name='pdbligand=CAC:'>CAC</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] Known structural/functional Site: <scene name='pdbsite=AC1:Cac+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BVT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: glycoside hydrolase]] | [[Category: glycoside hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:28:22 2008'' |
Revision as of 08:28, 3 February 2008
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THE STRUCTURE OF A MODULAR ENDO-BETA-1,4-MANNANASE FROM CELLULOMONAS FIMI EXPLAINS THE PRODUCT SPECIFICITY OF GLYCOSIDE HYDROLASE FAMILY 26 MANNANASES.
Overview
The endo-beta-1,4-mannanase from the soil bacterium Cellulomonas fimi is a, modular plant cell wall degrading enzyme involved in the hydrolysis of the, backbone of mannan, one of the most abundant polysaccharides of the, hemicellulosic network in the plant cell wall. The crystal structure of a, recombinant truncated endo-beta-1,4-mannanase from C. fimi (CfMan26A-50K), was determined by X-ray crystallography to 2.25 A resolution using the, molecular replacement technique. The overall structure of the enzyme, consists of a core (beta/alpha)8-barrel catalytic module characteristic of, clan GH-A, connected via a linker to an immunoglobulin-like module of, unknown function. A complex with the oligosaccharide mannotriose to 2.9 A, resolution has also been obtained. Both the native structure and the, complex show a cacodylate ion bound at the -1 subsite, while subsites -2, -3, and -4 are occupied by mannotriose in the complex. Enzyme kinetic, analysis and the analysis of hydrolysis products from, manno-oligosaccharides and mannopentitol suggest five important, active-site cleft subsites. CfMan26A-50K has a high affinity -3 subsite, with Phe325 as an aromatic platform, which explains the mannose releasing, property of the enzyme. Structural differences with the homologous, Cellvibrio japonicus beta-1,4-mannanase (CjMan26A) at the -2 and -3, subsites may explain the poor performance of CfMan26A mutants as, "glycosynthases".
About this Structure
2BVT is a Single protein structure of sequence from Cellulomonas fimi with as ligand. Active as Mannan endo-1,4-beta-mannosidase, with EC number 3.2.1.78 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The structure and characterization of a modular endo-beta-1,4-mannanase from Cellulomonas fimi., Le Nours J, Anderson L, Stoll D, Stalbrand H, Lo Leggio L, Biochemistry. 2005 Sep 27;44(38):12700-8. PMID:16171384
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