2c31
From Proteopedia
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| - | [[Image:2c31.gif|left|200px]]<br /><applet load="2c31" size=" | + | [[Image:2c31.gif|left|200px]]<br /><applet load="2c31" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2c31, resolution 1.73Å" /> | caption="2c31, resolution 1.73Å" /> | ||
'''CRYSTAL STRUCTURE OF OXALYL-COA DECARBOXYLASE IN COMPLEX WITH THE COFACTOR DERIVATIVE THIAMIN-2-THIAZOLONE DIPHOSPHATE AND ADENOSINE DIPHOSPHATE'''<br /> | '''CRYSTAL STRUCTURE OF OXALYL-COA DECARBOXYLASE IN COMPLEX WITH THE COFACTOR DERIVATIVE THIAMIN-2-THIAZOLONE DIPHOSPHATE AND ADENOSINE DIPHOSPHATE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2C31 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oxalobacter_formigenes Oxalobacter formigenes] with MG, TZD and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Oxalyl-CoA_decarboxylase Oxalyl-CoA decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.8 4.1.1.8] Known structural/functional Site: <scene name='pdbsite=AC1:Adp Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2C31 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oxalobacter_formigenes Oxalobacter formigenes] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=TZD:'>TZD</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Oxalyl-CoA_decarboxylase Oxalyl-CoA decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.8 4.1.1.8] Known structural/functional Site: <scene name='pdbsite=AC1:Adp+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C31 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: thiamine pyrophosphate]] | [[Category: thiamine pyrophosphate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:30:49 2008'' |
Revision as of 08:30, 3 February 2008
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CRYSTAL STRUCTURE OF OXALYL-COA DECARBOXYLASE IN COMPLEX WITH THE COFACTOR DERIVATIVE THIAMIN-2-THIAZOLONE DIPHOSPHATE AND ADENOSINE DIPHOSPHATE
Overview
Oxalyl-coenzyme A decarboxylase is a thiamin diphosphate-dependent enzyme, that plays an important role in the catabolism of the highly toxic, compound oxalate. We have determined the crystal structure of the enzyme, from Oxalobacter formigenes from a hemihedrally twinned crystal to 1.73 A, resolution and characterized the steady-state kinetic behavior of the, decarboxylase. The monomer of the tetrameric enzyme consists of three, alpha/beta-type domains, commonly seen in this class of enzymes, and the, thiamin diphosphate-binding site is located at the expected, subunit-subunit interface between two of the domains with the cofactor, bound in the conserved V-conformation. Although oxalyl-CoA decarboxylase, is structurally homologous to acetohydroxyacid synthase, a molecule of ADP, is bound in a region that is cognate to the FAD-binding site observed in, acetohydroxyacid synthase and presumably fulfils a similar role in, stabilizing the protein structure. This difference between the two enzymes, may have physiological importance since oxalyl-CoA decarboxylation is an, essential step in ATP generation in O. formigenes, and the decarboxylase, activity is stimulated by exogenous ADP. Despite the significant degree of, structural conservation between the two homologous enzymes and the, similarity in catalytic mechanism to other thiamin diphosphate-dependent, enzymes, the active site residues of oxalyl-CoA decarboxylase are unique., A suggestion for the reaction mechanism of the enzyme is presented.
About this Structure
2C31 is a Single protein structure of sequence from Oxalobacter formigenes with , and as ligands. Active as Oxalyl-CoA decarboxylase, with EC number 4.1.1.8 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis for activation of the thiamin diphosphate-dependent enzyme oxalyl-CoA decarboxylase by adenosine diphosphate., Berthold CL, Moussatche P, Richards NG, Lindqvist Y, J Biol Chem. 2005 Dec 16;280(50):41645-54. Epub 2005 Oct 10. PMID:16216870
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