2c37
From Proteopedia
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| - | [[Image:2c37.gif|left|200px]]<br /><applet load="2c37" size=" | + | [[Image:2c37.gif|left|200px]]<br /><applet load="2c37" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2c37, resolution 2.80Å" /> | caption="2c37, resolution 2.80Å" /> | ||
'''RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH U8 RNA'''<br /> | '''RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH U8 RNA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2C37 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with CL, NA, U5P, U5P and RP5 as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 2C37 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb86_1.html Exosomes]]. Known structural/functional Site: <scene name='pdbsite=AC1:U5p Binding Site For Chain X'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2C37 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=U5P:'>U5P</scene>, <scene name='pdbligand=U5P:'>U5P</scene> and <scene name='pdbligand=RP5:'>RP5</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 2C37 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb86_1.html Exosomes]]. Known structural/functional Site: <scene name='pdbsite=AC1:U5p+Binding+Site+For+Chain+X'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C37 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rrp42]] | [[Category: rrp42]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:30:51 2008'' |
Revision as of 08:30, 3 February 2008
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RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH U8 RNA
Overview
The exosome is a macromolecular complex that plays fundamental roles in, the biogenesis and turnover of a large number of RNA species. Here we, report the crystal structures of the Rrp41-Rrp42 core complex of the S., solfataricus exosome bound to short single-stranded RNAs and to ADP. The, RNA binding cleft recognizes four nucleotides in a sequence-unspecific, manner, mainly by electrostatic interactions with the phosphate groups., Interactions at the 2' hydroxyls of the sugars provide specificity for RNA, over DNA. The structures show both the bound substrate and the cleaved, product of the reaction, suggesting a catalytic mechanism for the 3'-5', phosphorolytic activity of the exosome.
About this Structure
2C37 is a Protein complex structure of sequences from Sulfolobus solfataricus with , , , and as ligands. The following page contains interesting information on the relation of 2C37 with [Exosomes]. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core., Lorentzen E, Conti E, Mol Cell. 2005 Nov 11;20(3):473-81. PMID:16285928
Page seeded by OCA on Sun Feb 3 10:30:51 2008
Categories: Exosomes | Protein complex | Sulfolobus solfataricus | Conti, E. | Lorentzen, E. | CL | NA | RP5 | U5P | Archaeal | Exoribonuclease | Exosome | Hydrolase | Phosphorolytic | Rna degradation | Rnase ph | Rrp41 | Rrp42
