2c4h
From Proteopedia
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| - | [[Image:2c4h.gif|left|200px]]<br /><applet load="2c4h" size=" | + | [[Image:2c4h.gif|left|200px]]<br /><applet load="2c4h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2c4h, resolution 2.15Å" /> | caption="2c4h, resolution 2.15Å" /> | ||
'''TORPEDO CALIFORNICA ACETYLCHOLINESTERASE IN COMPLEX WITH 500MM ACETYLTHIOCHOLINE'''<br /> | '''TORPEDO CALIFORNICA ACETYLCHOLINESTERASE IN COMPLEX WITH 500MM ACETYLTHIOCHOLINE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2C4H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica] with NAG, NDG, CL, ACT, PGE, AT3 and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] Known structural/functional Site: <scene name='pdbsite=AC1:Cl Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2C4H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=NDG:'>NDG</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=PGE:'>PGE</scene>, <scene name='pdbligand=AT3:'>AT3</scene> and <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] Known structural/functional Site: <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: synapse]] | [[Category: synapse]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:31:22 2008'' |
Revision as of 08:31, 3 February 2008
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TORPEDO CALIFORNICA ACETYLCHOLINESTERASE IN COMPLEX WITH 500MM ACETYLTHIOCHOLINE
Overview
Acetylcholinesterase (AChE) terminates nerve-impulse transmission at, cholinergic synapses by rapid hydrolysis of the neurotransmitter, acetylcholine. Substrate traffic in AChE involves at least two binding, sites, the catalytic and peripheral anionic sites, which have been, suggested to be allosterically related and involved in substrate, inhibition. Here, we present the crystal structures of Torpedo californica, AChE complexed with the substrate acetylthiocholine, the product, thiocholine and a nonhydrolysable substrate analogue. These structures, provide a series of static snapshots of the substrate en route to the, active site and identify, for the first time, binding of substrate and, product at both the peripheral and active sites. Furthermore, they provide, structural insight into substrate inhibition in AChE at two different, substrate concentrations. Our structural data indicate that substrate, inhibition at moderate substrate concentration is due to choline exit, being hindered by a substrate molecule bound at the peripheral site. At, the higher concentration, substrate inhibition arises from prevention of, exit of acetate due to binding of two substrate molecules within the, active-site gorge.
About this Structure
2C4H is a Single protein structure of sequence from Torpedo californica with , , , , , and as ligands. Active as Acetylcholinesterase, with EC number 3.1.1.7 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural insights into substrate traffic and inhibition in acetylcholinesterase., Colletier JP, Fournier D, Greenblatt HM, Stojan J, Sussman JL, Zaccai G, Silman I, Weik M, EMBO J. 2006 Jun 21;25(12):2746-56. Epub 2006 Jun 8. PMID:16763558
Page seeded by OCA on Sun Feb 3 10:31:22 2008
Categories: Acetylcholinesterase | Single protein | Torpedo californica | Colletier, J.P. | Fournier, D. | Greenblatt, H.M. | Silman, I. | Sussman, J.L. | Weik, M. | Zaccai, G. | ACE | ACT | AT3 | CL | NAG | NDG | PGE | Alpha/beta hydrolase | Alternative splicing | Glycoprotein | Gpi-anchor | Hydrolase | Lipoprotein | Membrane | Neurotransmitter cleavage | Neurotransmitter degradation | Serine esterase | Substrate-inhibition | Synapse
