User:Luis E Ramirez-Tapia/Sandbox 4

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(New page: <applet load='1ema' size='450' frame='true' align='right' scene='Green_Fluorescent_Protein/1ema_gfp_default/2' caption='Green fluorescent protein complex with peptide-derived chromophore (...)
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<applet load='1ema' size='450' frame='true' align='right' scene='Green_Fluorescent_Protein/1ema_gfp_default/2' caption='Green fluorescent protein complex with peptide-derived chromophore ([[1ema]])' />
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{{Seed}}
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'''Green fluorescent protein (GFP)''' is a [[bioluminescent]] polypeptide consisting of 238 residues isolated from the body of [[Aequorea victoria]] jellyfish.<ref name="PDBsum">[http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1ema&template=main.html], Protein Database (PDBsum): 1ema. European Bioinformatics (EBI); 2009.</ref> GFP converts the blue chemiluminescent of [[aequorin]] in the jellyfish into green fluorescent light.<ref name="Yang">[http://www-bioc.rice.edu/Bioch/Phillips/Papers/gfpbio.html], Yang F, Moss LG, Phillips GN Jr. 1996. The molecular structure of green fluorescent protein. Biotechnology. 14: 1246-1251. DOI 10.1038/nbt1096-1246.</ref> It remains unclear why these jellyfish use fluorescence, why green is better than blue, or why they produce a separate protein for green fluorescence as opposed to simply mutating the present aequorin to shift its wavelength,<ref name="Tsien" /> but in the laboratory, GFP can be incorporated into a variety of biological systems in order to function as a marker protein. Since its discovery in 1962, GFP has become a significant contributor to the research of monitoring gene expression, localization, mobility, traffic, interactions between various membrane and cytoplasmic proteins, as well as many others.<ref name="Haldar">[http://www.springerlink.com/content/wvg513864266g77n/fulltext.pdf], Haldar S, Chattopadhyay A. 2009. The green journey. J Fluoresc. 19:1-2. DOI 10.1007/s10895-008-0455-6; biographical background on [http://en.wikipedia.org/wiki/Douglas_Prasher Douglas Prasher], [http://en.wikipedia.org/wiki/Martin_Chalfie Martin Chalfie] and [http://en.wikipedia.org/wiki/Roger_Tsien Roger Tsien].</ref>
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[[Image:1qoy.png|left|200px]]
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The line below this paragraph, containing "STRUCTURE_1qoy", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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{{STRUCTURE_1qoy| PDB=1qoy | SCENE= }}
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===E.COLI HEMOLYSIN E (HLYE, CLYA, SHEA)===
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{{ABSTRACT_PUBMED_10660049}}
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==About this Structure==
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1QOY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QOY OCA].
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==Reference==
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E. coli hemolysin E (HlyE, ClyA, SheA): X-ray crystal structure of the toxin and observation of membrane pores by electron microscopy., Wallace AJ, Stillman TJ, Atkins A, Jamieson SJ, Bullough PA, Green J, Artymiuk PJ, Cell. 2000 Jan 21;100(2):265-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10660049 10660049]
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[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Artymiuk, P J.]]
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[[Category: Atkins, A.]]
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[[Category: Bullough, P A.]]
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[[Category: Green, J.]]
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[[Category: Jamieson, S J.]]
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[[Category: Stillman, T J.]]
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[[Category: Wallace, A J.]]
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[[Category: Cytolysin]]
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[[Category: Hemolysin]]
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[[Category: Membrane pore former]]
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[[Category: Pore]]
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[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:22:40 2008''

Revision as of 17:39, 7 December 2011

Image:1qoy.png

Template:STRUCTURE 1qoy

E.COLI HEMOLYSIN E (HLYE, CLYA, SHEA)

Template:ABSTRACT PUBMED 10660049

About this Structure

1QOY is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

E. coli hemolysin E (HlyE, ClyA, SheA): X-ray crystal structure of the toxin and observation of membrane pores by electron microscopy., Wallace AJ, Stillman TJ, Atkins A, Jamieson SJ, Bullough PA, Green J, Artymiuk PJ, Cell. 2000 Jan 21;100(2):265-76. PMID:10660049

Page seeded by OCA on Tue Jul 29 12:22:40 2008

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Luis E Ramirez-Tapia

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