Phosphotransferase
From Proteopedia
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| - | {{STRUCTURE_3q2m| PDB=3q2m | SIZE= | + | {{STRUCTURE_3q2m| PDB=3q2m | SIZE=400| SCENE= |right| CAPTION=Aminoglycoside phosphotransferase complex with spectinomycin and Ni+2 ion, [[3q2m]] }} |
'''Phosphotransferase''' (PT) are enzymes which catalyze phosphorylation reactions. The acceptor group can be alcohol, carboxy, nitrogenous, phosphate or a pair ofd groups. Aminoglycoside PT (AGPT) inactivate aminoglycoside antibiotics by phosphorylation. The phosphoenolpyruvate-protein PT (PEP) catalyzes the phosphorylation of protein histidine residues. See [[Enzyme I of the Phosphoenolpyruvate:Sugar Phosphotransferase System]] for some details. | '''Phosphotransferase''' (PT) are enzymes which catalyze phosphorylation reactions. The acceptor group can be alcohol, carboxy, nitrogenous, phosphate or a pair ofd groups. Aminoglycoside PT (AGPT) inactivate aminoglycoside antibiotics by phosphorylation. The phosphoenolpyruvate-protein PT (PEP) catalyzes the phosphorylation of protein histidine residues. See [[Enzyme I of the Phosphoenolpyruvate:Sugar Phosphotransferase System]] for some details. | ||
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==3D structures of phosphotransferase== | ==3D structures of phosphotransferase== | ||
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[[3our]] – PT IIA + pyruvate decarboxylase – ''Vibrio vulnificus''<BR /> | [[3our]] – PT IIA + pyruvate decarboxylase – ''Vibrio vulnificus''<BR /> | ||
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[[2ooc]] – PT histidine – ''Caulobacter crescentus''<BR /> | [[2ooc]] – PT histidine – ''Caulobacter crescentus''<BR /> | ||
[[3ipj]] – PT IIABC – ''Clostridium difficile''<BR /> | [[3ipj]] – PT IIABC – ''Clostridium difficile''<BR /> | ||
| - | [[2f9h]] - | + | [[2f9h]] - EfPT IIA – ''Enterococcus faecalis''<BR /> |
| + | [[1c02]] - PT - yeast | ||
===Aminoglycoside phosphotransferase=== | ===Aminoglycoside phosphotransferase=== | ||
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[[3q2m]], [[3i1a]] – LpAGPT IA – ''Legionella pneumophila''<BR /> | [[3q2m]], [[3i1a]] – LpAGPT IA – ''Legionella pneumophila''<BR /> | ||
[[1nd4]] – KpAGPT IIA – ''Klebsiella pneumoniae''<BR /> | [[1nd4]] – KpAGPT IIA – ''Klebsiella pneumoniae''<BR /> | ||
| - | [[1qhn]] – SvAGPT – Streptomyces venezuelae<BR /> | + | [[1qhn]] – SvAGPT – ''Streptomyces venezuelae''<BR /> |
[[3sgc]], [[3r7z]], [[3n4t]], [[3n4u]], [[3n4v]] - EncAGPT ID – ''Enterococcus casseliflavus''<BR /> | [[3sgc]], [[3r7z]], [[3n4t]], [[3n4u]], [[3n4v]] - EncAGPT ID – ''Enterococcus casseliflavus''<BR /> | ||
| - | [[1j7i]] - | + | [[1j7i]] - EfAGPT IIIA |
===Aminoglycoside phosphotransferase complex with nucleotide=== | ===Aminoglycoside phosphotransferase complex with nucleotide=== | ||
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[[1qhx]] - SvAGPT + ATP<BR /> | [[1qhx]] - SvAGPT + ATP<BR /> | ||
[[3i0q]] - LpAGPT IA + AMP<BR /> | [[3i0q]] - LpAGPT IA + AMP<BR /> | ||
| - | [[1j7l]] - | + | [[1j7l]] - EfAGPT IIIA + ADP <BR /> |
| - | [[1j7u]] - | + | [[1j7u]] - EfAGPT IIIA + AMP-PNP |
===Aminoglycoside phosphotransferase complex with antibiotic<br />=== | ===Aminoglycoside phosphotransferase complex with antibiotic<br />=== | ||
| - | [[3hav]], [[3ham]] - EfAGPT + ATP + antibiotic | + | [[3hav]], [[3ham]] - EfAGPT + ATP + antibiotic <BR /> |
| - | [[3h8p]] - EfAGPT + AMP-PNP + antibiotic<br /> | + | [[3h8p]], [[3tm0]] - EfAGPT + AMP-PNP + antibiotic<br /> |
[[2b0q]], [[1l8t]] - EfAGPT IIIA + ADP + antibiotic<br /> | [[2b0q]], [[1l8t]] - EfAGPT IIIA + ADP + antibiotic<br /> | ||
[[3r80]], [[3r81]] - EcAGPT ID + antibiotic<br /> | [[3r80]], [[3r81]] - EcAGPT ID + antibiotic<br /> | ||
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===Aminoglycoside phosphotransferase complex with inhibitor=== | ===Aminoglycoside phosphotransferase complex with inhibitor=== | ||
| - | [[ | + | [[2bkk]], [[3q2j]] - EfAGPT IIIA + inhibitor |
| - | [[ | + | |
===Phosphoenolpyruvate-protein phosphotransferase=== | ===Phosphoenolpyruvate-protein phosphotransferase=== | ||
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[[1wcr]] - EcPEP diacetylchitobiose-specific IIA (mutant) – NMR<BR /> | [[1wcr]] - EcPEP diacetylchitobiose-specific IIA (mutant) – NMR<BR /> | ||
[[1h9c]] - EcPEP diacetylchitobiose-specific IIB – NMR<BR /> | [[1h9c]] - EcPEP diacetylchitobiose-specific IIB – NMR<BR /> | ||
| + | [[2hwg]] - EcPEP | ||
[[3ipr]] – EfPEP gluconate-specific IIA<BR /> | [[3ipr]] – EfPEP gluconate-specific IIA<BR /> | ||
[[3bed]] - EfPEP mannose-specific IIA<BR /> | [[3bed]] - EfPEP mannose-specific IIA<BR /> | ||
| - | [[1nrz]] - KpPEP sorbose-specific IIB | + | [[1nrz]] - KpPEP sorbose-specific IIB<br /> |
| + | [[2hro]] – PEP – ''Staphylococcus carnosus''<br /> | ||
| + | [[2wqd]] - PEP (mutant) – ''Staphylococcus aureus''<br /> | ||
| + | [[3trc]] - PEP (mutant) – ''Coxiella burnetii'' | ||
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| + | [[1f51]], [[1pey]], [[2ftk]] – BsPT sporulation initiation<br /> | ||
| + | [[1pux]] - BsPT sporulation initiation – NMR<br /> | ||
| + | [[2jvi]], [[2jvj]], [[2jvk]] - BsPT sporulation initiation (mutant) – NMR<br /> | ||
| + | [[3q15]] - BsPT sporulation initiation + response regulator aspartate phosphatase<br /> | ||
| + | [[1fyn]] – PT SH3 domain + polyproline peptide - human | ||
Revision as of 12:06, 8 December 2011
Template:STRUCTURE 3q2m
Phosphotransferase (PT) are enzymes which catalyze phosphorylation reactions. The acceptor group can be alcohol, carboxy, nitrogenous, phosphate or a pair ofd groups. Aminoglycoside PT (AGPT) inactivate aminoglycoside antibiotics by phosphorylation. The phosphoenolpyruvate-protein PT (PEP) catalyzes the phosphorylation of protein histidine residues. See Enzyme I of the Phosphoenolpyruvate:Sugar Phosphotransferase System for some details.
3D structures of phosphotransferase
Updated December 2011
3our – PT IIA + pyruvate decarboxylase – Vibrio vulnificus
2l5h, 2kx9 - EcPT I - Escherichia coli – NMR
2xdf - EcPT I+ phosphocarrier protein HPR – NMR
3oxp – PT II – Yersinia pestis
3ci6 – PT GAF domain – Acinetobacter
2ooc – PT histidine – Caulobacter crescentus
3ipj – PT IIABC – Clostridium difficile
2f9h - EfPT IIA – Enterococcus faecalis
1c02 - PT - yeast
Aminoglycoside phosphotransferase
3ovc - EcAGPT IA
3r6z - EcAGPT IB
3q2m, 3i1a – LpAGPT IA – Legionella pneumophila
1nd4 – KpAGPT IIA – Klebsiella pneumoniae
1qhn – SvAGPT – Streptomyces venezuelae
3sgc, 3r7z, 3n4t, 3n4u, 3n4v - EncAGPT ID – Enterococcus casseliflavus
1j7i - EfAGPT IIIA
Aminoglycoside phosphotransferase complex with nucleotide
3r70 - EcAGPT IB + ADP
3r78 - EcAGPT IB + ATP
1qhx - SvAGPT + ATP
3i0q - LpAGPT IA + AMP
1j7l - EfAGPT IIIA + ADP
1j7u - EfAGPT IIIA + AMP-PNP
Aminoglycoside phosphotransferase complex with antibiotic
3hav, 3ham - EfAGPT + ATP + antibiotic
3h8p, 3tm0 - EfAGPT + AMP-PNP + antibiotic
2b0q, 1l8t - EfAGPT IIIA + ADP + antibiotic
3r80, 3r81 - EcAGPT ID + antibiotic
1grq, 1grr, 1qhs - SvAGPT + antibiotic
1qhy - SvAGPT + ATPγ + antibiotic
3sg8, 3sg9 - EncAGPT ID + antibiotic
3i0o - LpAGPT IA + ADP + antibiotic
Aminoglycoside phosphotransferase complex with inhibitor
2bkk, 3q2j - EfAGPT IIIA + inhibitor
Phosphoenolpyruvate-protein phosphotransferase
2xz7 – TtPEP PEP-binding domain + phosphoenolpyruvate – Thermoanaerobacter tengcongensis
2xz9 - TtPEP PEP-binding domain + pyruvate
3nbm – PEP lactose-specific IIBC – Streptococcus pneumoniae
2l2q - PEP cellbiose-specific IIB – Borrelia burgdorferi – NMR
3k1s - PEP cellbiose-specific IIA – Bacillus anthracis
2kyr – EcPEP fructose-specific IIB
2r4q - BsPEP fructose-specific IIABC – Bacillus subtilis
2r48 - BsPEP fructose-specific IIB
1vsq, 2jzh, 2jzn, 2jzo - EcPEP mannose-specific IIA - NMR
3bp3 - EcPEP glucose-specific IIB
1o53 - EcPEP glucose-specific IIA N terminal – NMR
1ggr - EcPEP glucose-specific IIA + phosphocarrier protein HPR – NMR
3bp8 - EcPEP glucose-specific IIB + NAGC-like transcriptional regulator
3eye - EcPEP acetylgalactosamine-specific IIB
2oq3, 1vrv - EcPEP mannitol-specific IIA
1vkr - EcPEP mannitol-specific IIABC – NMR
1j6t - EcPEP mannitol-specific IIABC + phosphocarrier protein HPR – NMR
2few - EcPEP mannitol-specific IIA (mutant)
1wcr - EcPEP diacetylchitobiose-specific IIA (mutant) – NMR
1h9c - EcPEP diacetylchitobiose-specific IIB – NMR
2hwg - EcPEP
3ipr – EfPEP gluconate-specific IIA
3bed - EfPEP mannose-specific IIA
1nrz - KpPEP sorbose-specific IIB
2hro – PEP – Staphylococcus carnosus
2wqd - PEP (mutant) – Staphylococcus aureus
3trc - PEP (mutant) – Coxiella burnetii
1f51, 1pey, 2ftk – BsPT sporulation initiation
1pux - BsPT sporulation initiation – NMR
2jvi, 2jvj, 2jvk - BsPT sporulation initiation (mutant) – NMR
3q15 - BsPT sporulation initiation + response regulator aspartate phosphatase
1fyn – PT SH3 domain + polyproline peptide - human
