2c9k
From Proteopedia
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| - | [[Image:2c9k.gif|left|200px]]<br /><applet load="2c9k" size=" | + | [[Image:2c9k.gif|left|200px]]<br /><applet load="2c9k" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2c9k, resolution 2.8Å" /> | caption="2c9k, resolution 2.8Å" /> | ||
'''STRUCTURE OF THE FUNCTIONAL FORM OF THE MOSQUITO-LARVICIDAL CRY4AA TOXIN FROM BACILLUS THURINGIENSIS AT 2.8 A RESOLUTION'''<br /> | '''STRUCTURE OF THE FUNCTIONAL FORM OF THE MOSQUITO-LARVICIDAL CRY4AA TOXIN FROM BACILLUS THURINGIENSIS AT 2.8 A RESOLUTION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2C9K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis] with MPD as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Mpd Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2C9K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis] with <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Mpd+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C9K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: sporulation]] | [[Category: sporulation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:33:08 2008'' |
Revision as of 08:33, 3 February 2008
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STRUCTURE OF THE FUNCTIONAL FORM OF THE MOSQUITO-LARVICIDAL CRY4AA TOXIN FROM BACILLUS THURINGIENSIS AT 2.8 A RESOLUTION
Overview
The Cry4Aa delta-endotoxin from Bacillus thuringiensis is toxic to larvae, of Culex, Anopheles, and Aedes mosquitoes, which are vectors of important, human tropical diseases. With the objective of designing modified toxins, with improved potency that could be used as biopesticides, we determined, the structure of this toxin in its functional form at a resolution of 2.8, angstroms. Like other Cry delta-endotoxins, the activated Cry4Aa toxin, consists of three globular domains, a seven-alpha-helix bundle responsible, for pore formation (domain I) and the following two other domains having, structural similarities with carbohydrate binding proteins: a beta-prism, (domain II) and a plant lectin-like beta-sandwich (domain III). We also, studied the effect on toxicity of amino acid substitutions and deletions, in three loops located at the surface of the putative receptor binding, domain II of Cry4Aa. Our results indicate that one loop is an important, determinant of toxicity, presumably through attachment of Cry4Aa to the, surface of mosquito cells. The availability of the Cry4Aa structure should, guide further investigations aimed at the molecular basis of the target, specificity and membrane insertion of Cry endotoxins.
About this Structure
2C9K is a Single protein structure of sequence from Bacillus thuringiensis with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of the functional form of the mosquito larvicidal Cry4Aa toxin from Bacillus thuringiensis at a 2.8-angstrom resolution., Boonserm P, Mo M, Angsuthanasombat C, Lescar J, J Bacteriol. 2006 May;188(9):3391-401. PMID:16621834
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