2cbf
From Proteopedia
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| - | [[Image:2cbf.gif|left|200px]]<br /><applet load="2cbf" size=" | + | [[Image:2cbf.gif|left|200px]]<br /><applet load="2cbf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2cbf, resolution 3.1Å" /> | caption="2cbf, resolution 3.1Å" /> | ||
'''THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF'''<br /> | '''THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2CBF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium] with SAH as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] Known structural/functional Sites: <scene name='pdbsite=FLP:Two Regions Contain Significantly Altered Locations Rela ...'>FLP</scene> and <scene name='pdbsite=SAH:S-Adenosyl-Homocysteine Binding Site. Crystals Formed In ...'>SAH</scene>. Full crystallographic information is available from [http:// | + | 2CBF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium] with <scene name='pdbligand=SAH:'>SAH</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] Known structural/functional Sites: <scene name='pdbsite=FLP:Two+Regions+Contain+Significantly+Altered+Locations+Rela+...'>FLP</scene> and <scene name='pdbsite=SAH:S-Adenosyl-Homocysteine+Binding+Site.+Crystals+Formed+In+...'>SAH</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: precorrin-4 methyltransferase]] | [[Category: precorrin-4 methyltransferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:33:36 2008'' |
Revision as of 08:33, 3 February 2008
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THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF
Overview
Biosynthesis of the corrin ring of vitamin B12 requires the action of six, S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely, related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been, determined to a resolution of 2.4 A. CbiF contains two alphabeta domains, forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The, location of AdoHcy and a number of conserved residues, helps define the, precorrin binding site. A second crystal form determined at 3.1 A, resolution highlights the flexibility of two loops around this site. CbiF, employs a unique mode of AdoHcy binding and represents a new class of, transmethylase.
About this Structure
2CBF is a Single protein structure of sequence from Bacillus megaterium with as ligand. Active as Precorrin-4 C(11)-methyltransferase, with EC number 2.1.1.133 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase., Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ, Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:9665173
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